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Literature summary extracted from

  • Avigad, G.; Alroy, Y.; Englard, S.
    Purification and properties of a nicotinamide adenine dinucleotide phosphate-linked aldohexose dehydrogenase from Gluconobacter cerinus (1968), J. Biol. Chem., 243, 1936-1941.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.119 freezing and thawing: significant loss of activity Gluconobacter cerinus
1.1.1.119 lyophilization of dilute enzyme solutions, below 0.5 ng/ml, results in significant loss of activity Gluconobacter cerinus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.119 CuSO4
-
 Gluconobacter cerinus
1.1.1.119 additional information overview: sugars found to be neither substrates nor inhibitors Gluconobacter cerinus
1.1.1.119 NADPH competitive to NADP+ Gluconobacter cerinus
1.1.1.119 ZnSO4
-
 Gluconobacter cerinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.119 0.012
-
 NADP+
-
 Gluconobacter cerinus
1.1.1.119 0.038
-
 NADPH
-
 Gluconobacter cerinus
1.1.1.119 1.8
-
 D-mannose in 0.1 M Tris-buffer pH 8.0 Gluconobacter cerinus
1.1.1.119 5.3
-
 D-glucose
-
 Gluconobacter cerinus
1.1.1.119 5.6
-
 D-mannose in 0.1 M potassium phosphate pH 6.2 Gluconobacter cerinus
1.1.1.119 29
-
 2-amino-2-deoxy-D-mannose
-
 Gluconobacter cerinus
1.1.1.119 39
-
 2-deoxy-D-glucose
-
 Gluconobacter cerinus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.119 Gluconobacter cerinus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.119 partial Gluconobacter cerinus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.119 10.1
-
-
 Gluconobacter cerinus

Storage Stability

EC Number Storage Stability Organism
1.1.1.119 -20°C, 1 year Gluconobacter cerinus
1.1.1.119 4°C, pH 5.4 - pH 8.5, 8 weeks Gluconobacter cerinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.119 2-amino-2-deoxy-D-mannose + NADP+
-
 Gluconobacter cerinus 2-amino-2-deoxy-D-mannono-1,5-lactone + NADPH
-
 ?
1.1.1.119 2-deoxy-D-glucose + NADP+
-
 Gluconobacter cerinus 2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.119 D-glucose + NADP+
-
 Gluconobacter cerinus D-glucono-1,5-lactone + NADPH + H+
-
 r
1.1.1.119 D-mannose + NADP+
-
 Gluconobacter cerinus D-mannono-1,5-lactone + NADPH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.119 8
-
 in Tris-buffer Gluconobacter cerinus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.119 7.5 9 broad range of optimal activity, in Tris-buffer consistently higher than that in phosphate-buffers Gluconobacter cerinus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.119 NADP+
-
 Gluconobacter cerinus
1.1.1.119 NADPH
-
 Gluconobacter cerinus