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Literature summary extracted from

  • Anderson, R.L.; Dahms, A.S.
    D-Aldohexose dehydrogenase (1975), Methods Enzymol., 41, 147-150.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.118 0.86
-
 D-glucose
-
 Pseudomonas sp.
1.1.1.118 5.8
-
 D-fucose
-
 Pseudomonas sp.
1.1.1.118 80
-
 NAD+
-
 Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.118 Pseudomonas sp.
-
 MSU-1
-
1.1.1.118 Pseudomonas sp. MSU-1
-
 MSU-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.118
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.118 66
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.118 stable to freezing for at least 6 months, pH 7.0, 10 mM sodium phosphate buffer Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.118 2-deoxy-D-galactose + NAD+
-
 Pseudomonas sp. 2-deoxy-D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.118 2-deoxy-D-glucose + NAD+
-
 Pseudomonas sp. 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 2-deoxy-D-glucose + NAD+
-
 Pseudomonas sp. MSU-1 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 3,6-dideoxy-D-galactose + NAD+
-
 Pseudomonas sp. 3,6-dideoxy-D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.118 6-deoxy-D-galactose + NAD+
-
 Pseudomonas sp. 6-deoxy-D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.118 6-deoxy-D-glucose + NAD+
-
 Pseudomonas sp. 6-deoxy-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 D-allose + NAD+
-
 Pseudomonas sp. D-allono-1,5-lactone + NADH
-
 ?
1.1.1.118 D-altrose + NAD+
-
 Pseudomonas sp. D-altrono-1,5-lactone + NADH
-
 ?
1.1.1.118 D-fucose + NAD+
-
 Pseudomonas sp. D-fucono-1,5-lactone + NADH + H+
-
 r
1.1.1.118 D-galactose + NAD+
-
 Pseudomonas sp. D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.118 D-galactose + NAD+
-
 Pseudomonas sp. MSU-1 D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.118 D-glucose + NAD+
-
 Pseudomonas sp. D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 D-glucose + NAD+
-
 Pseudomonas sp. MSU-1 D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.118 D-mannose + NAD+
-
 Pseudomonas sp. D-manno-1,5-lactone + NADH
-
 ?
1.1.1.118 D-mannose + NAD+
-
 Pseudomonas sp. MSU-1 D-manno-1,5-lactone + NADH
-
 ?
1.1.1.118 additional information no activity with: D-glucuronic acid, D-galacturonic acid, D-galactose 6-phosphate, D-glucose 6-phosphate, D-glucosamine, N-acetyl-D-glucosamine, 6-deoxy-D-allose, 6-iodo-6-deoxy-D-galactose, 2-acetamide-6-deoxy-D-allose, 2-acetamido-6-deoxy-D-altrose Pseudomonas sp. ?
-
 ?
1.1.1.118 additional information no activity with: D-glucuronic acid, D-galacturonic acid, D-galactose 6-phosphate, D-glucose 6-phosphate, D-glucosamine, N-acetyl-D-glucosamine, 6-deoxy-D-allose, 6-iodo-6-deoxy-D-galactose, 2-acetamide-6-deoxy-D-allose, 2-acetamido-6-deoxy-D-altrose Pseudomonas sp. MSU-1 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.118 8 8.5 Tris-HCl buffer Pseudomonas sp.
1.1.1.118 9 10 glycine-NaOH buffer Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.118 NAD+ cofactor Pseudomonas sp.