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Literature summary extracted from

  • Neuberger, M.S.; Patterson, R.A.; Hartley, B.S.
    Purification and properties of Klebsiella aerogenes D-arabitol dehydrogenase (1979), Biochem. J., 183, 31-42.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.11 gene over-expressed in Escherichia coli K12 Klebsiella aerogenes

General Stability

EC Number General Stability Organism
1.1.1.11 glycerol stabilizes Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.11 0.04
-
 NAD+
-
 Klebsiella aerogenes
1.1.1.11 20
-
 D-arabinitol
-
 Klebsiella aerogenes
1.1.1.11 70
-
 D-mannitol
-
 Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.11 43000
-
 gel filtration, ultracentrifugation Klebsiella aerogenes
1.1.1.11 44000
-
 sedimentation equilibrium Klebsiella aerogenes
1.1.1.11 46000
-
 calculated from amino acid composition Klebsiella aerogenes
1.1.1.11 46500
-
 1 * 46500, SDS-PAGE Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.11 D-arabinitol + NAD+ Klebsiella aerogenes
-
 D-xylulose + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.11 Klebsiella aerogenes
-
 i.e. Klebsiella pneumoniae, gene expression in Escherichia coli K12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.11 recombinant protein from Escherichia coli Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.11 37.1
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
1.1.1.11 -20°C, protein concentration 50 mg/ml, 50% v/v glycerol, stable 3 months, loss of activity less than 50% Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.11 D-arabinitol + NAD+
-
 Klebsiella aerogenes D-xylulose + NADH + H+
-
 ?
1.1.1.11 D-mannitol + NAD+
-
 Klebsiella aerogenes D-fructose + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.11 monomer 1 * 46500, SDS-PAGE Klebsiella aerogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.11 28
-
 enzyme assay at Klebsiella aerogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.11 45
-
 20 min Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.11 NAD+
-
 Klebsiella aerogenes