EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.106 | 0.033 | - |
D-pantoate | at pH 10.0 | Pseudomonas sp. P2 | |
1.1.1.106 | 0.5 | - |
ketopantoate | - |
Pseudomonas sp. P2 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.106 | (R)-pantoate + NAD+ | Pseudomonas sp. P2 | - |
(R)-4-dehydropantoate + NADH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.106 | Pseudomonas sp. P2 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.106 | 50°C, protamine sulfate, calcium phosphate gel, ammonium sulfate, 50°C | Pseudomonas sp. P2 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.106 | 6 | - |
- |
Pseudomonas sp. P2 |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.106 | -20°C, 2 mM potassium phosphate, pH 7.0, 0.01% EDTA, 1 mM mercaptoethanol, several months, no loss of activity | Pseudomonas sp. P2 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.106 | (R)-pantoate + NAD+ | - |
Pseudomonas sp. P2 | (R)-4-dehydropantoate + NADH | - |
r | |
1.1.1.106 | 2-oxo-pantoate + NAD+ | - |
Pseudomonas sp. P2 | 2-oxo-4-dehydropantoate | - |
? |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.106 | 7 | 10 | linear activity increase | Pseudomonas sp. P2 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.106 | NAD+ | - |
Pseudomonas sp. P2 |