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Literature summary extracted from

  • Shimakata, T.; Stumpf, P.K.
    Fatty acid synthetase of Spinacia oleracea leaves (1982), Plant Physiol., 69, 1257-1262.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 additional information
-
 additional information
-
 Spinacia oleracea
1.1.1.100 0.015
-
 NADPH
-
 Spinacia oleracea
1.1.1.100 0.017
-
 acetoacetyl-[acyl-carrier protein]
-
 Spinacia oleracea
1.3.1.9 0.0014
-
 crotonyl-[acyl-carrier protein] reductase I Spinacia oleracea
1.3.1.9 0.003
-
 NADH reductase I Spinacia oleracea
1.3.1.9 0.003
-
 2-decenoyl-[acyl-carrier protein] reductase I Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.100 64000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH Spinacia oleracea enzyme in fatty acid synthesis (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Spinacia oleracea
-
-
-
1.3.1.9 Spinacia oleracea
-
 var. Viroflay
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.100
-
 Spinacia oleracea
1.3.1.9 two different forms I and II Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.100 leaf
-
 Spinacia oleracea
-
1.3.1.9 leaf no reductase I activity in crude extracts Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.9 additional information
-
 specific activity of reductase I and II incrude extracts Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
1.3.1.9 -20°C, at least 3 months without loss of activity Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Spinacia oleracea (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH enzyme in fatty acid synthesis Spinacia oleracea (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 acetoacetyl-[acyl-carrier protein] + NADPH
-
 Spinacia oleracea D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
 ?
1.3.1.9 2-decenoyl-[acyl-carrier protein] + NADH
-
 Spinacia oleracea decanoyl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 crotonyl-[acyl-carrier protein] + NADH
-
 Spinacia oleracea butyryl-[acyl-carrier protein] + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.100 5.7 6.2
-
 Spinacia oleracea
1.3.1.9 6.1
-
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADH NADPH is much more effective than NADH Spinacia oleracea
1.1.1.100 NADPH much more effective than NADH Spinacia oleracea
1.3.1.9 NADH reductase I Spinacia oleracea