BRENDA - Enzyme Database

Purification and characterizations of beta-ketoacyl-[acyl-carrier-protein] reductase, beta-hydroxyacyl-[acylcarrier-protein] dehydrase, and enoyl-[acyl-carrier-protein] reductase from Spinacia oleracea leaves

Shimakata, T.; Stumpf, P.K.; Arch. Biochem. Biophys. 218, 77-91 (1982) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.100
additional information
not: N-ethylmaleimide, arsenite
Spinacia oleracea
1.1.1.100
p-chloromercuribenzoate
-
Spinacia oleracea
1.3.1.9
additional information
not inhibitory: arsenite
Spinacia oleracea
1.3.1.9
N-ethylmaleimide
not
Spinacia oleracea
1.3.1.9
p-chloromercuribenzoate
87% inhibition at 0.1 mM
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.100
0.0037
-
acetoacetyl-[acyl-carrier protein]
-
Spinacia oleracea
1.1.1.100
0.025
-
NADPH
-
Spinacia oleracea
1.1.1.100
0.25
-
acetoacetyl-CoA
-
Spinacia oleracea
1.3.1.9
0.0029
-
2-hexadecenoyl-[acyl-carrier protein]
-
Spinacia oleracea
1.3.1.9
0.00552
-
2-decenoyl-[acyl-carrier protein]
-
Spinacia oleracea
1.3.1.9
0.0062
-
2-hexenoyl-[acyl-carrier protein]
-
Spinacia oleracea
1.3.1.9
0.0097
-
crotonyl-[acyl-carrier protein]
-
Spinacia oleracea
1.3.1.9
0.011
-
NADH
-
Spinacia oleracea
1.3.1.9
0.0133
-
2-octenoyl-[acyl-carrier protein]
-
Spinacia oleracea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.100
24200
-
4 * 24200, SDS-PAGE
Spinacia oleracea
1.1.1.100
97000
-
gel filtration
Spinacia oleracea
1.3.1.9
32500
-
monomeric form, SDS-PAGE
Spinacia oleracea
1.3.1.9
115000
-
gel filtration
Spinacia oleracea
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.100
Escherichia coli
-
-
-
1.1.1.100
Spinacia oleracea
-
-
-
1.3.1.9
Spinacia oleracea
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.100
-
Spinacia oleracea
1.3.1.9
homogeneity
Spinacia oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.100
leaf
-
Spinacia oleracea
-
1.3.1.9
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.100
0.0032
-
-
Escherichia coli
1.1.1.100
0.005
-
-
Spinacia oleracea
1.1.1.100
7.95
-
-
Spinacia oleracea
1.3.1.9
63.69
-
-
Spinacia oleracea
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.100
-20°C, stable for 2 months
Spinacia oleracea
1.3.1.9
-20°C, 2 months
Spinacia oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.100
3-oxoacyl-[acyl-carrier protein] + NADPH
-
285668
Escherichia coli
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
285668
Escherichia coli
r
1.1.1.100
3-oxoacyl-[acyl-carrier protein] + NADPH
-
285668
Spinacia oleracea
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
285668
Spinacia oleracea
r
1.1.1.100
acetoacetyl-CoA + NADPH
-
285668
Escherichia coli
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
?
1.1.1.100
acetoacetyl-CoA + NADPH
-
285668
Spinacia oleracea
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
?
1.1.1.100
acetoacetyl-[acyl-carrier protein] + NADPH
-
285668
Escherichia coli
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
285668
Escherichia coli
r
1.1.1.100
acetoacetyl-[acyl-carrier protein] + NADPH
-
285668
Spinacia oleracea
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
285668
Spinacia oleracea
r
1.1.1.100
additional information
L-isomer completely inactive
285668
Escherichia coli
?
-
-
-
?
1.1.1.100
additional information
L-isomer completely inactive
285668
Spinacia oleracea
?
-
-
-
?
1.3.1.9
2-decenoyl-[acyl-carrier protein] + NADH
-
285668
Spinacia oleracea
decanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
2-dodecenoyl-[acyl-carrier protein] + NADH
-
285668
Spinacia oleracea
dodecanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
2-hexadecenoyl-[acyl-carrier protein] + NADH
-
285668
Spinacia oleracea
hexadecanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
2-hexenoyl-[acyl-carrier protein] + NADH
fast reduction
285668
Spinacia oleracea
hexanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
2-octenoyl-[acyl-carrier protein] + NADH
fast reduction
285668
Spinacia oleracea
octanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
crotonyl-[acyl-carrier protein] + NADH
-
285668
Spinacia oleracea
butyryl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
additional information
no reduction of 3-decenoyl-[acyl-carrier-protein]
285668
Spinacia oleracea
?
-
-
-
?
1.3.1.9
additional information
substrate specificity: acyl chain length C4-C16
285668
Spinacia oleracea
?
-
-
-
?
1.3.1.9
trans-2-decenoyl-[acyl-carrier protein] + NADH
-
285668
Spinacia oleracea
decanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.100
tetramer
4 * 24200, SDS-PAGE
Spinacia oleracea
1.3.1.9
tetramer
4 * 32500, SDS-PAGE
Spinacia oleracea
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.1.9
6.4
-
-
Spinacia oleracea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.1.9
6.4
-
-
Spinacia oleracea
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.100
6
8.7
both reaction directions
Spinacia oleracea
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.100
NADH
NADPH is much more effective than NADH
Spinacia oleracea
1.1.1.100
NADP+
-
Escherichia coli
1.1.1.100
NADP+
-
Spinacia oleracea
1.1.1.100
NADPH
-
Escherichia coli
1.1.1.100
NADPH
-
Spinacia oleracea
1.3.1.9
NADH
-
Spinacia oleracea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.100
NADH
NADPH is much more effective than NADH
Spinacia oleracea
1.1.1.100
NADP+
-
Escherichia coli
1.1.1.100
NADP+
-
Spinacia oleracea
1.1.1.100
NADPH
-
Escherichia coli
1.1.1.100
NADPH
-
Spinacia oleracea
1.3.1.9
NADH
-
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.100
additional information
not: N-ethylmaleimide, arsenite
Spinacia oleracea
1.1.1.100
p-chloromercuribenzoate
-
Spinacia oleracea
1.3.1.9
additional information
not inhibitory: arsenite
Spinacia oleracea
1.3.1.9
N-ethylmaleimide
not
Spinacia oleracea
1.3.1.9
p-chloromercuribenzoate
87% inhibition at 0.1 mM
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.100
0.0037
-
acetoacetyl-[acyl-carrier protein]
-
Spinacia oleracea
1.1.1.100
0.025
-
NADPH
-
Spinacia oleracea
1.1.1.100
0.25
-
acetoacetyl-CoA
-
Spinacia oleracea
1.3.1.9
0.0029
-
2-hexadecenoyl-[acyl-carrier protein]
-
Spinacia oleracea
1.3.1.9
0.00552
-
2-decenoyl-[acyl-carrier protein]
-
Spinacia oleracea
1.3.1.9
0.0062
-
2-hexenoyl-[acyl-carrier protein]
-
Spinacia oleracea
1.3.1.9
0.0097
-
crotonyl-[acyl-carrier protein]
-
Spinacia oleracea
1.3.1.9
0.011
-
NADH
-
Spinacia oleracea
1.3.1.9
0.0133
-
2-octenoyl-[acyl-carrier protein]
-
Spinacia oleracea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.100
24200
-
4 * 24200, SDS-PAGE
Spinacia oleracea
1.1.1.100
97000
-
gel filtration
Spinacia oleracea
1.3.1.9
32500
-
monomeric form, SDS-PAGE
Spinacia oleracea
1.3.1.9
115000
-
gel filtration
Spinacia oleracea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.100
-
Spinacia oleracea
1.3.1.9
homogeneity
Spinacia oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.100
leaf
-
Spinacia oleracea
-
1.3.1.9
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.100
0.0032
-
-
Escherichia coli
1.1.1.100
0.005
-
-
Spinacia oleracea
1.1.1.100
7.95
-
-
Spinacia oleracea
1.3.1.9
63.69
-
-
Spinacia oleracea
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.100
-20°C, stable for 2 months
Spinacia oleracea
1.3.1.9
-20°C, 2 months
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.100
3-oxoacyl-[acyl-carrier protein] + NADPH
-
285668
Escherichia coli
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
285668
Escherichia coli
r
1.1.1.100
3-oxoacyl-[acyl-carrier protein] + NADPH
-
285668
Spinacia oleracea
(3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
285668
Spinacia oleracea
r
1.1.1.100
acetoacetyl-CoA + NADPH
-
285668
Escherichia coli
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
?
1.1.1.100
acetoacetyl-CoA + NADPH
-
285668
Spinacia oleracea
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
?
1.1.1.100
acetoacetyl-[acyl-carrier protein] + NADPH
-
285668
Escherichia coli
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
285668
Escherichia coli
r
1.1.1.100
acetoacetyl-[acyl-carrier protein] + NADPH
-
285668
Spinacia oleracea
D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
285668
Spinacia oleracea
r
1.1.1.100
additional information
L-isomer completely inactive
285668
Escherichia coli
?
-
-
-
?
1.1.1.100
additional information
L-isomer completely inactive
285668
Spinacia oleracea
?
-
-
-
?
1.3.1.9
2-decenoyl-[acyl-carrier protein] + NADH
-
285668
Spinacia oleracea
decanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
2-dodecenoyl-[acyl-carrier protein] + NADH
-
285668
Spinacia oleracea
dodecanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
2-hexadecenoyl-[acyl-carrier protein] + NADH
-
285668
Spinacia oleracea
hexadecanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
2-hexenoyl-[acyl-carrier protein] + NADH
fast reduction
285668
Spinacia oleracea
hexanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
2-octenoyl-[acyl-carrier protein] + NADH
fast reduction
285668
Spinacia oleracea
octanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
crotonyl-[acyl-carrier protein] + NADH
-
285668
Spinacia oleracea
butyryl-[acyl-carrier protein] + NAD+
-
-
-
?
1.3.1.9
additional information
no reduction of 3-decenoyl-[acyl-carrier-protein]
285668
Spinacia oleracea
?
-
-
-
?
1.3.1.9
additional information
substrate specificity: acyl chain length C4-C16
285668
Spinacia oleracea
?
-
-
-
?
1.3.1.9
trans-2-decenoyl-[acyl-carrier protein] + NADH
-
285668
Spinacia oleracea
decanoyl-[acyl-carrier protein] + NAD+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.100
tetramer
4 * 24200, SDS-PAGE
Spinacia oleracea
1.3.1.9
tetramer
4 * 32500, SDS-PAGE
Spinacia oleracea
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.1.9
6.4
-
-
Spinacia oleracea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.1.9
6.4
-
-
Spinacia oleracea
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.100
6
8.7
both reaction directions
Spinacia oleracea