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Literature summary extracted from

  • Witteveen, C.F.B.; Weber, F.; Busink, R.; Visser, J.
    Isolation and characterization of two xylitol dehydrogenases from Aspergillus niger (1994), Microbiology, 140, 1679-1685.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.10 0.03
-
 NADPH
-
 Aspergillus niger
1.1.1.10 0.13
-
 NADP+
-
 Aspergillus niger
1.1.1.10 17
-
 L-xylulose
-
 Aspergillus niger
1.1.1.10 925
-
 xylitol
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.10 32000
-
 8 * 32000, SDS-PAGE Aspergillus niger
1.1.1.10 250000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.10 L-xylulose + NADPH + H+ Aspergillus niger
-
 L-xylitol + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.10 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.10
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.10 33
-
 xylitol oxidation, NADP+ Aspergillus niger
1.1.1.10 200
-
 L-xylulose reduction, NADPH Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
1.1.1.10 4°C, Tris-HCl 20 mM, pH 7.0, at leat several weeks Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.10 D-sorbitol + NADP+
-
 Aspergillus niger ?
-
 ?
1.1.1.10 L-xylulose + NADPH + H+
-
 Aspergillus niger L-xylitol + NADP+
-
 r
1.1.1.10 additional information minimal reductive activity also with D-xylulose, D-erythrose and dihydroxyacetone Aspergillus niger ?
-
 ?
1.1.1.10 xylitol + NADP+
-
 Aspergillus niger L-xylulose + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.10 octamer 8 * 32000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
1.1.1.10 More xylitol:NAD+ 2-oxidoreductase, EC 1.1.1.9, is termed as xylitol dehydrogenase Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.10 6.5
-
 reduction of L-xylulose Aspergillus niger
1.1.1.10 10 11 oxidation of xylitol Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.10 5 8.5 reduction of L-xylulose Aspergillus niger
1.1.1.10 8 11.5 oxidation of xylitol Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.10 additional information determination of Ki of NADP+ and NADPH in competition with each other Aspergillus niger
1.1.1.10 NAD+ no activity with Aspergillus niger
1.1.1.10 NADP+
-
 Aspergillus niger
1.1.1.10 NADPH preference for Aspergillus niger