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Literature summary extracted from

  • Rella, R.; Raia, C.A.; Pensa, M.; Pisani, F.M.; Gambacorta, A.; de Rosa, M.; Rossi, M.
    A novel archaebacterial NAD+-dependent alcohol dehydrogenase. Purification and properties (1987), Eur. J. Biochem., 167, 475-479.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 EDTA 15 mM, 85% inhibition Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.0025
-
 Anisaldehyde
-
 Saccharolobus solfataricus
1.1.1.1 0.013
-
 benzyl alcohol
-
 Saccharolobus solfataricus
1.1.1.1 0.019
-
 propan-1-ol
-
 Saccharolobus solfataricus
1.1.1.1 0.021
-
 2-Methylcyclohexanol
-
 Saccharolobus solfataricus
1.1.1.1 0.05
-
 pentan-2-ol
-
 Saccharolobus solfataricus
1.1.1.1 0.055
-
 butan-1-ol
-
 Saccharolobus solfataricus
1.1.1.1 0.225
-
 pentan-3-ol
-
 Saccharolobus solfataricus
1.1.1.1 0.25
-
 (+/-)-3-methylcyclohexanone
-
 Saccharolobus solfataricus
1.1.1.1 0.26
-
 ethanol
-
 Saccharolobus solfataricus
1.1.1.1 0.35
-
 butan-2-ol
-
 Saccharolobus solfataricus
1.1.1.1 0.5
-
 propan-2-ol
-
 Saccharolobus solfataricus
1.1.1.1 1.3
-
 Cyclopentanone
-
 Saccharolobus solfataricus
1.1.1.1 6.6
-
 acetone
-
 Saccharolobus solfataricus
1.1.1.1 7.5
-
 3-methylbutan-2-one
-
 Saccharolobus solfataricus
1.1.1.1 14
-
 butan-2-one
-
 Saccharolobus solfataricus
1.1.1.1 27
-
 methanol
-
 Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zinc 2 mM required for optimal activity Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 37000
-
 2 * 37000, SDS-PAGE Saccharolobus solfataricus
1.1.1.1 70000
-
 gel filtration, sucrose density gradient centrifugation Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 3.92
-
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 2-methylcyclohexanol + NAD+
-
 Saccharolobus solfataricus (+)-2-methylcyclohexanone + NADH + H+
-
 r
1.1.1.1 3-methylbutan-2-one + NADH
-
 Saccharolobus solfataricus 3-methylbutan-2-ol + NAD+
-
 ?
1.1.1.1 3-methylcyclohexanol + NAD+
-
 Saccharolobus solfataricus 3-methylcyclohexanone + NADH
-
 r
1.1.1.1 3-methylcyclohexanone + NADH
-
 Saccharolobus solfataricus 3-methylcyclohexanol + NAD+
-
 r
1.1.1.1 acetone + NADH
-
 Saccharolobus solfataricus isopropanol + NAD+
-
 r
1.1.1.1 anisaldehyde + NADH + H+
-
 Saccharolobus solfataricus anisic alcohol + NAD+
-
 ?
1.1.1.1 benzyl alcohol + NAD+
-
 Saccharolobus solfataricus benzaldehyde + NADH
-
 ?
1.1.1.1 butan-2-ol + NAD+
-
 Saccharolobus solfataricus butan-2-one + NADH
-
 ?
1.1.1.1 butan-2-one + NADH
-
 Saccharolobus solfataricus butan-2-ol + NAD+
-
 r
1.1.1.1 butanol + NAD+
-
 Saccharolobus solfataricus butyraldehyde + NADH
-
 ?
1.1.1.1 cyclopetanone + NADH + H+
-
 Saccharolobus solfataricus cyclopentanol + NAD+
-
 ?
1.1.1.1 ethanol + NAD+
-
 Saccharolobus solfataricus acetaldehyde + NADH + H+
-
 ?
1.1.1.1 methanol + NAD+
-
 Saccharolobus solfataricus formaldehyde + NADH + H+
-
 r
1.1.1.1 pentan-2-ol + NAD+
-
 Saccharolobus solfataricus 2-pentanone + NADH
-
 ?
1.1.1.1 pentan-3-ol + NAD+
-
 Saccharolobus solfataricus 3-pentanone + NADH
-
 ?
1.1.1.1 propan-2-ol + NAD+
-
 Saccharolobus solfataricus acetone + NADH
-
 ?
1.1.1.1 propanol + NAD+
-
 Saccharolobus solfataricus propionaldehyde + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 37000, SDS-PAGE Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 45 95 continous increase in activity from 40°C to 95°C Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 50
-
 24 h, 30% loss of activity Saccharolobus solfataricus
1.1.1.1 60
-
 half-life: 20 h Saccharolobus solfataricus
1.1.1.1 70
-
 half-life: 5 h Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 0.317
-
 acetone
-
 Saccharolobus solfataricus
1.1.1.1 0.983
-
 Anisaldehyde
-
 Saccharolobus solfataricus
1.1.1.1 1.02
-
 Cyclopentanone
-
 Saccharolobus solfataricus
1.1.1.1 1.22
-
 3-methylbutan-2-one
-
 Saccharolobus solfataricus
1.1.1.1 1.68
-
 benzyl alcohol
-
 Saccharolobus solfataricus
1.1.1.1 1.88
-
 ethanol
-
 Saccharolobus solfataricus
1.1.1.1 1.93
-
 butan-2-one
-
 Saccharolobus solfataricus
1.1.1.1 1.97
-
 butan-1-ol
-
 Saccharolobus solfataricus
1.1.1.1 2.17
-
 3-methylcyclohexanone
-
 Saccharolobus solfataricus
1.1.1.1 2.92
-
 pentan-3-ol
-
 Saccharolobus solfataricus
1.1.1.1 3.1
-
 methanol
-
 Saccharolobus solfataricus
1.1.1.1 3.55
-
 butan-2-ol
-
 Saccharolobus solfataricus
1.1.1.1 3.82
-
 pentan2-ol
-
 Saccharolobus solfataricus
1.1.1.1 4.03
-
 propan-1-ol
-
 Saccharolobus solfataricus
1.1.1.1 6.92
-
 propan-2-ol
-
 Saccharolobus solfataricus
1.1.1.1 7.72
-
 3-methyl-cyclohexanol
-
 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 7.5
-
 reduction of anisaldehyde Saccharolobus solfataricus
1.1.1.1 8.5
-
 oxidation of benzyl alcohol Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Saccharolobus solfataricus
1.1.1.1 NADH
-
 Saccharolobus solfataricus