BRENDA - Enzyme Database

Biochemical properties of alcohol dehydrogenase from Drosophila lebanonensis

Winberg, J.O.; Hovik, R.; McKinley-McKee, J.S.; Juan, E.; Gonzalez-Duarte, R.; Biochem. J. 235, 481-490 (1986) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
2,2,2-Trifluoroethanol
competitive towards ethanol
Scaptodrosophila lebanonensis
1.1.1.1
2-Chloroethanol
competitive towards ethanol
Scaptodrosophila lebanonensis
1.1.1.1
Cibacron blue
competitive towards NAD+
Scaptodrosophila lebanonensis
1.1.1.1
imidazole
pure competitive inhibition towards ethanol and (R)-(+)-phenylethanol
Scaptodrosophila lebanonensis
1.1.1.1
isoburyramide
competitive towards ethanol and butan-2-ol
Scaptodrosophila lebanonensis
1.1.1.1
NADH
competitive towards NAD+
Scaptodrosophila lebanonensis
1.1.1.1
pyrazole
competitive towards ethanol
Scaptodrosophila lebanonensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.011
-
NAD+
oxidation of ethanol
Scaptodrosophila lebanonensis
1.1.1.1
0.028
-
NAD+
oxidation of propan-2-ol
Scaptodrosophila lebanonensis
1.1.1.1
0.72
-
Cyclohexanol
-
Scaptodrosophila lebanonensis
1.1.1.1
0.81
-
4-methylpentan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
0.85
-
S-(-)-trans-bicyclo(2.2.1)-heptanol
-
Scaptodrosophila lebanonensis
1.1.1.1
0.95
-
S-(-)-2-methylbutan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
1
-
cis-4-methylcyclohexanol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.1
-
2-propanol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.1
-
trans-4-methylcyclohexanol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.2
-
R-(+)-cis-verbenol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.5
-
R-(+)-trans-bicyclo(2.2.1)-heptanol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.9
-
butan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.9
-
propan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
2.7
-
2-methylpropan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
3.4
-
R-(+)-1-phenylethanol
-
Scaptodrosophila lebanonensis
1.1.1.1
3.8
-
S-(-)-cis-bicyclo(2.2.1)-heptanol
-
Scaptodrosophila lebanonensis
1.1.1.1
3.9
-
S-(-)-1-phenylethanol
-
Scaptodrosophila lebanonensis
1.1.1.1
4.1
-
pentan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
6
-
ethanol
-
Scaptodrosophila lebanonensis
1.1.1.1
6.2
-
ethanol
-
Scaptodrosophila lebanonensis
1.1.1.1
6.6
-
heptan-4-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
7.4
-
hexan-1-ol
-
Scaptodrosophila lebanonensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
28000
-
2 * 28000, SDS-PAGE
Scaptodrosophila lebanonensis
1.1.1.1
56000
-
gel filtration
Scaptodrosophila lebanonensis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.1
Scaptodrosophila lebanonensis
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.1
(S)-2-methylbutan-1-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
(S)-2-methyl-butanal + NADH + H+
-
-
-
?
1.1.1.1
2-methylbutan-1-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
? + NADH
-
-
-
?
1.1.1.1
2-methylpropan-1-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
2-methyl-propan-1-one + NADH
-
-
-
?
1.1.1.1
3-methylbutan-2-ol + NAD+
R-(-)-3-methylbutan-2-ol and S-(+)-3-methylbutan-2-ol
285616
Scaptodrosophila lebanonensis
3-methylbutan-2-one + NADH
-
-
-
?
1.1.1.1
4-methyl-1-pentanol + NAD+
-
285616
Scaptodrosophila lebanonensis
4-methyl-1-pentanal + NADH
-
-
-
?
1.1.1.1
butan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
butan-2-one + NADH
-
-
-
?
1.1.1.1
butanol + NAD+
-
285616
Scaptodrosophila lebanonensis
butyraldehyde + NADH
-
-
-
?
1.1.1.1
cis-4-methylcyclohexanol + NAD+
-
285616
Scaptodrosophila lebanonensis
4-methylcyclohexanone + NADH
-
-
-
?
1.1.1.1
cyclohexanol + NAD+
-
285616
Scaptodrosophila lebanonensis
cyclohexanone + NADH
-
-
-
?
1.1.1.1
ethanol + NAD+
-
285616
Scaptodrosophila lebanonensis
acetaldehyde + NADH + H+
-
-
-
r
1.1.1.1
ethanol + NAD+
100% activity
285616
Scaptodrosophila lebanonensis
acetaldehyde + NADH + H+
-
-
-
r
1.1.1.1
heptan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
heptan-2-one + NADH
-
-
-
?
1.1.1.1
heptan-4-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
heptan-4-one + NADH
-
-
-
?
1.1.1.1
hexan-1-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
n-hexanal + NADH
-
-
-
?
1.1.1.1
hexan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
2-hexanone + NADH
-
-
-
?
1.1.1.1
octan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
octan-2-one + NADH
-
-
-
?
1.1.1.1
pentan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
2-pentanone + NADH
-
-
-
?
1.1.1.1
pentanol + NAD+
-
285616
Scaptodrosophila lebanonensis
n-pentanal + NADH
-
-
-
?
1.1.1.1
phenylethanol + NAD+
R-(+)-phenylethanol and S-(-)-phenylethanol
285616
Scaptodrosophila lebanonensis
phenylacetaldehyde + NADH
-
-
-
?
1.1.1.1
propan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
acetone + NADH
-
-
-
?
1.1.1.1
propanol + NAD+
-
285616
Scaptodrosophila lebanonensis
propionaldehyde + NADH + H+
-
-
-
?
1.1.1.1
R-(+)-1-phenylethanol + NAD+
-
285616
Scaptodrosophila lebanonensis
1-phenylethanone + NADH
-
-
-
?
1.1.1.1
R-(+)-cis-verbenol + NAD+
-
285616
Scaptodrosophila lebanonensis
? + NADH
-
-
-
?
1.1.1.1
R-(+)-trans-bicyclo(2.2.1)-heptanol + NAD+
R-(+)-trans-bicyclo(2.2.1)-heptanol and S-(-)-trans-bicyclo(2.2.1)-heptanol
285616
Scaptodrosophila lebanonensis
R-(+)-trans-bicyclo(2.2.1)-heptanal + NADH
-
-
-
?
1.1.1.1
S-(-)-1-phenylethanol + NAD+
-
285616
Scaptodrosophila lebanonensis
1-phenylethanone + NADH
-
-
-
?
1.1.1.1
S-(-)-cis-bicyclo(2.2.1)-heptanol + NAD+
-
285616
Scaptodrosophila lebanonensis
S-(-)-cis-bicyclo(2.2.1)-heptanal + NADH
-
-
-
?
1.1.1.1
S-(-)-trans-bicyclo(2.2.1)-heptanol + NAD+
1054% activity compared to ethanol
285616
Scaptodrosophila lebanonensis
S-(-)-cis-bicyclo(2.2.1)-heptanal + NADH + H+
-
-
-
r
1.1.1.1
trans-4-methylcyclohexanol + NAD+
-
285616
Scaptodrosophila lebanonensis
4-methylcyclohexanone + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.1
dimer
2 * 28000, SDS-PAGE
Scaptodrosophila lebanonensis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Scaptodrosophila lebanonensis
1.1.1.1
NADH
-
Scaptodrosophila lebanonensis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Scaptodrosophila lebanonensis
1.1.1.1
NADH
-
Scaptodrosophila lebanonensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
2,2,2-Trifluoroethanol
competitive towards ethanol
Scaptodrosophila lebanonensis
1.1.1.1
2-Chloroethanol
competitive towards ethanol
Scaptodrosophila lebanonensis
1.1.1.1
Cibacron blue
competitive towards NAD+
Scaptodrosophila lebanonensis
1.1.1.1
imidazole
pure competitive inhibition towards ethanol and (R)-(+)-phenylethanol
Scaptodrosophila lebanonensis
1.1.1.1
isoburyramide
competitive towards ethanol and butan-2-ol
Scaptodrosophila lebanonensis
1.1.1.1
NADH
competitive towards NAD+
Scaptodrosophila lebanonensis
1.1.1.1
pyrazole
competitive towards ethanol
Scaptodrosophila lebanonensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.011
-
NAD+
oxidation of ethanol
Scaptodrosophila lebanonensis
1.1.1.1
0.028
-
NAD+
oxidation of propan-2-ol
Scaptodrosophila lebanonensis
1.1.1.1
0.72
-
Cyclohexanol
-
Scaptodrosophila lebanonensis
1.1.1.1
0.81
-
4-methylpentan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
0.85
-
S-(-)-trans-bicyclo(2.2.1)-heptanol
-
Scaptodrosophila lebanonensis
1.1.1.1
0.95
-
S-(-)-2-methylbutan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
1
-
cis-4-methylcyclohexanol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.1
-
2-propanol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.1
-
trans-4-methylcyclohexanol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.2
-
R-(+)-cis-verbenol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.5
-
R-(+)-trans-bicyclo(2.2.1)-heptanol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.9
-
butan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
1.9
-
propan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
2.7
-
2-methylpropan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
3.4
-
R-(+)-1-phenylethanol
-
Scaptodrosophila lebanonensis
1.1.1.1
3.8
-
S-(-)-cis-bicyclo(2.2.1)-heptanol
-
Scaptodrosophila lebanonensis
1.1.1.1
3.9
-
S-(-)-1-phenylethanol
-
Scaptodrosophila lebanonensis
1.1.1.1
4.1
-
pentan-1-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
6
-
ethanol
-
Scaptodrosophila lebanonensis
1.1.1.1
6.2
-
ethanol
-
Scaptodrosophila lebanonensis
1.1.1.1
6.6
-
heptan-4-ol
-
Scaptodrosophila lebanonensis
1.1.1.1
7.4
-
hexan-1-ol
-
Scaptodrosophila lebanonensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
28000
-
2 * 28000, SDS-PAGE
Scaptodrosophila lebanonensis
1.1.1.1
56000
-
gel filtration
Scaptodrosophila lebanonensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.1
(S)-2-methylbutan-1-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
(S)-2-methyl-butanal + NADH + H+
-
-
-
?
1.1.1.1
2-methylbutan-1-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
? + NADH
-
-
-
?
1.1.1.1
2-methylpropan-1-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
2-methyl-propan-1-one + NADH
-
-
-
?
1.1.1.1
3-methylbutan-2-ol + NAD+
R-(-)-3-methylbutan-2-ol and S-(+)-3-methylbutan-2-ol
285616
Scaptodrosophila lebanonensis
3-methylbutan-2-one + NADH
-
-
-
?
1.1.1.1
4-methyl-1-pentanol + NAD+
-
285616
Scaptodrosophila lebanonensis
4-methyl-1-pentanal + NADH
-
-
-
?
1.1.1.1
butan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
butan-2-one + NADH
-
-
-
?
1.1.1.1
butanol + NAD+
-
285616
Scaptodrosophila lebanonensis
butyraldehyde + NADH
-
-
-
?
1.1.1.1
cis-4-methylcyclohexanol + NAD+
-
285616
Scaptodrosophila lebanonensis
4-methylcyclohexanone + NADH
-
-
-
?
1.1.1.1
cyclohexanol + NAD+
-
285616
Scaptodrosophila lebanonensis
cyclohexanone + NADH
-
-
-
?
1.1.1.1
ethanol + NAD+
-
285616
Scaptodrosophila lebanonensis
acetaldehyde + NADH + H+
-
-
-
r
1.1.1.1
ethanol + NAD+
100% activity
285616
Scaptodrosophila lebanonensis
acetaldehyde + NADH + H+
-
-
-
r
1.1.1.1
heptan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
heptan-2-one + NADH
-
-
-
?
1.1.1.1
heptan-4-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
heptan-4-one + NADH
-
-
-
?
1.1.1.1
hexan-1-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
n-hexanal + NADH
-
-
-
?
1.1.1.1
hexan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
2-hexanone + NADH
-
-
-
?
1.1.1.1
octan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
octan-2-one + NADH
-
-
-
?
1.1.1.1
pentan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
2-pentanone + NADH
-
-
-
?
1.1.1.1
pentanol + NAD+
-
285616
Scaptodrosophila lebanonensis
n-pentanal + NADH
-
-
-
?
1.1.1.1
phenylethanol + NAD+
R-(+)-phenylethanol and S-(-)-phenylethanol
285616
Scaptodrosophila lebanonensis
phenylacetaldehyde + NADH
-
-
-
?
1.1.1.1
propan-2-ol + NAD+
-
285616
Scaptodrosophila lebanonensis
acetone + NADH
-
-
-
?
1.1.1.1
propanol + NAD+
-
285616
Scaptodrosophila lebanonensis
propionaldehyde + NADH + H+
-
-
-
?
1.1.1.1
R-(+)-1-phenylethanol + NAD+
-
285616
Scaptodrosophila lebanonensis
1-phenylethanone + NADH
-
-
-
?
1.1.1.1
R-(+)-cis-verbenol + NAD+
-
285616
Scaptodrosophila lebanonensis
? + NADH
-
-
-
?
1.1.1.1
R-(+)-trans-bicyclo(2.2.1)-heptanol + NAD+
R-(+)-trans-bicyclo(2.2.1)-heptanol and S-(-)-trans-bicyclo(2.2.1)-heptanol
285616
Scaptodrosophila lebanonensis
R-(+)-trans-bicyclo(2.2.1)-heptanal + NADH
-
-
-
?
1.1.1.1
S-(-)-1-phenylethanol + NAD+
-
285616
Scaptodrosophila lebanonensis
1-phenylethanone + NADH
-
-
-
?
1.1.1.1
S-(-)-cis-bicyclo(2.2.1)-heptanol + NAD+
-
285616
Scaptodrosophila lebanonensis
S-(-)-cis-bicyclo(2.2.1)-heptanal + NADH
-
-
-
?
1.1.1.1
S-(-)-trans-bicyclo(2.2.1)-heptanol + NAD+
1054% activity compared to ethanol
285616
Scaptodrosophila lebanonensis
S-(-)-cis-bicyclo(2.2.1)-heptanal + NADH + H+
-
-
-
r
1.1.1.1
trans-4-methylcyclohexanol + NAD+
-
285616
Scaptodrosophila lebanonensis
4-methylcyclohexanone + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.1
dimer
2 * 28000, SDS-PAGE
Scaptodrosophila lebanonensis