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Literature summary extracted from

  • Algar, E.M.; Seeley, T.L.; Holmes, R.S.
    Purification and molecular properties of mouse alcohol dehydrogenase isozymes (1983), Eur. J. Biochem., 137, 139-147.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 1,10-phenanthroline inhibition of isoenzyme A2 and C2, no inhibition of isoenzyme B2 Mus musculus
1.1.1.1 pyrazole inhibition of isoenzyme A2 and C2. Isoenzyme B2 is insensitive to pyrazole inhibition with trans-2-hexen-1-ol as substrate Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zinc isoenzyme A2 contains 2.7 mol of zinc per mol of enzyme, isoenzyme b2 contains 1.9 mol of zinc per mol of enzyme, isoenzyme C2 contains 3.2 mol of zinc per mol of enzyme. A and C subunits each contain two atoms of zinc, with at least one being involved catalytically, the b subunit probably contains a single non-catalytic zinc atom Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 39000
-
 2 * 39000, isoenzyme B2, SDS-PAGE Mus musculus
1.1.1.1 43000
-
 2 * 43000, isoenzyme A2, SDS-PAGE Mus musculus
1.1.1.1 47000
-
 2 * 47000, isoenzyme C2, SDS-PAGE Mus musculus
1.1.1.1 79000
-
 isoenzyme B2, gel filtration Mus musculus
1.1.1.1 83000
-
 isoenzyme A2, gel filtration Mus musculus
1.1.1.1 85000
-
 isoenzyme C2, gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.1 ethanol + NAD+ Mus musculus role of the major liver isoenzyme A2 in ethanol metabolism acetaldehyde + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Mus musculus
-
 isoenzyme A2, B2 and C2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 liver isoenzyme A2 and B2 and stomach isoenzyme C2 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.1 liver isoenzyme A2 and B2 Mus musculus
-
1.1.1.1 stomach isoenzyme C2 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 1.36
-
 isoenzyme A2 Mus musculus
1.1.1.1 8.08
-
 isoenzyme B2 Mus musculus
1.1.1.1 64
-
 isoenzyme C2 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 (E)-hex-2-en-1-ol + NAD+
-
 Mus musculus (E)-hex-2-en-1-one + NADH
-
 ?
1.1.1.1 acetaldehyde + NADH + H+ reduced by isoenzyme A2 and C2, no activity with isoenzyme B2 Mus musculus ethanol + NAD+
-
 ?
1.1.1.1 benzaldehyde + NADH reduced by isoenzyme A2 and C2, no activity with isoenzyme B2 Mus musculus benzyl alcohol + NAD+
-
 r
1.1.1.1 benzyl alcohol + NAD+ oxidized by isoenzyme A2 and C2 no activity with isoenzyme B2 Mus musculus benzaldehyde + NADH
-
 ?
1.1.1.1 cyclohexanol + NAD+ oxidized by isoenzyme A2, no activity with isoenzymes B2 and C2 Mus musculus cyclohexanone + NADH
-
 ?
1.1.1.1 ethanol + NAD+ no activity with isoenzyme B2, oxidized by isoenzyme A2 and C2 Mus musculus acetaldehyde + NADH
-
 ?
1.1.1.1 ethanol + NAD+ role of the major liver isoenzyme A2 in ethanol metabolism Mus musculus acetaldehyde + NADH
-
 ?
1.1.1.1 ethylene glycol + NAD+ oxidized by isoenzyme A2, no activity with isoenzyme B2 and C2 Mus musculus ? + NADH
-
 ?
1.1.1.1 formaldehyde + NADH + H+ reduced by isoenzyme A2, no activity with isoenzyme B2 and C2 Mus musculus methanol + NAD+
-
 r
1.1.1.1 hexanol + NAD+
-
 Mus musculus n-hexanal + NADH
-
 ?
1.1.1.1 methanol + NAD+ weak activity with isoenzyme A2, no activity with isoenzyme B2 and C2 Mus musculus formaldehyde + NADH + H+
-
 ?
1.1.1.1 methanol + NAD+ weak activity with isoenzyme A2, no activity with isoenzyme B2 and C2 Mus musculus formaldehyde + NADH + H+
-
 r
1.1.1.1 octan-1-ol + NAD+
-
 Mus musculus n-octanal + NADH
-
 ?
1.1.1.1 p-nitrobenzaldehyde + NADH
-
 Mus musculus p-nitrobenzyl alcohol + NAD+
-
 ?
1.1.1.1 pentanal + NAD+ oxidized by isoenzyme A2 and B2, no activity with isoenzyme C2 Mus musculus pentanone + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 47000, isoenzyme C2, SDS-PAGE Mus musculus
1.1.1.1 dimer 2 * 39000, isoenzyme B2, SDS-PAGE Mus musculus
1.1.1.1 dimer 2 * 43000, isoenzyme A2, SDS-PAGE Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Mus musculus
1.1.1.1 NADH
-
 Mus musculus