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Literature summary extracted from

  • Wagner, F.W.; Pares, X.; Holmquist, B.; Vallee, B.L.
    Physical and enzymatic properties of a class III isozyme of human liver alcohol dehydrogenase: chi-ADH (1984), Biochemistry, 23, 2193-2199.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 4-Methylpyrazole class III enzyme is completely insensitive to inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.0025
-
 NADH
-
 Homo sapiens
1.1.1.1 0.0064
-
 NADH isoenzyme beta1,beta1 Homo sapiens
1.1.1.1 0.007
-
 NADH isoenzyme gamma1,gamma1 Homo sapiens
1.1.1.1 0.0074
-
 NAD+ isoenzyme beta2,beta2 Homo sapiens
1.1.1.1 0.0079
-
 NAD+ isoenzyme gamma1,gamma1 Homo sapiens
1.1.1.1 0.0087
-
 NAD+ isoenzyme gamma2,gamma2 Homo sapiens
1.1.1.1 0.011
-
 NADH isoenzyme alpha,alpha Homo sapiens
1.1.1.1 0.013
-
 NAD+ isoenzyme alpha,alpha Homo sapiens
1.1.1.1 0.025
-
 NAD+
-
 Homo sapiens
1.1.1.1 0.1
-
 12-hydroxydodecanoate
-
 Homo sapiens
1.1.1.1 0.105
-
 NADH isoenzyme beta2,beta2 Homo sapiens
1.1.1.1 0.18
-
 NAD+ isoenzyme beta1,beta1 Homo sapiens
1.1.1.1 1.2
-
 Octanol
-
 Homo sapiens
1.1.1.1 2.4
-
 octanal
-
 Homo sapiens
1.1.1.1 11
-
 Vanillyl alcohol
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zinc contains 3.8 mol of Zn per mol of protein Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 40000
-
 2 * 40000, SDS-PAGE Homo sapiens
1.1.1.1 41000
-
 2 * 41000, class III isoenzyme chi ADH, SDS-PAGE Homo sapiens
1.1.1.1 82700
-
 equilibrium sedimentation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Homo sapiens
-
 isoenzyme chi-ADH
-
1.1.1.1 Homo sapiens
-
 class III isoenzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 class III isoenzyme chi-ADH Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.1 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 0.6
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.1.1.1 4°C, 10 mM HEPES buffer, 1 mM dithioerythritol, pH 7.5, stable for 2 weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 12-hydroxydodecanoate + NAD+
-
 Homo sapiens 12-oxododecanoic acid + NADH
-
 ?
1.1.1.1 17beta-hydroxyetiocholan-3-one + NAD+
-
 Homo sapiens ethiocholan-3,17-dione + NADH
-
 ?
1.1.1.1 benzyl alcohol + NAD+
-
 Homo sapiens benzaldehyde + NADH
-
 ?
1.1.1.1 butanol + NAD+
-
 Homo sapiens butyraldehyde + NADH
-
 ?
1.1.1.1 digitose + NAD+
-
 Homo sapiens ? + NADH
-
 ?
1.1.1.1 ethanol + NAD+ class III isoenzyme chi-ADH oxidizes ethanol very poorly Homo sapiens acetaldehyde + NADH
-
 ?
1.1.1.1 ethylene glycol + NAD+ class III isoenzyme chi-ADH shows no activity Homo sapiens ? + NADH
-
 ?
1.1.1.1 furfuryl alcohol + NAD+
-
 Homo sapiens furfural + NADH
-
 ?
1.1.1.1 methanol + NAD+ class III isoenzyme chi-ADH shows no activity Homo sapiens formaldehyde + NADH + H+
-
 ?
1.1.1.1 octan-2-ol + NAD+
-
 Homo sapiens octan-2-one + NADH
-
 ?
1.1.1.1 octanal + NADH + H+
-
 Homo sapiens octanol + NAD+
-
 ?
1.1.1.1 pentanal + NAD+
-
 Homo sapiens pentanone + NADH
-
 ?
1.1.1.1 phenylalaninol + NAD+
-
 Homo sapiens ? + NADH
-
 ?
1.1.1.1 vanillyl alcohol + NAD+
-
 Homo sapiens vanillin + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 40000, SDS-PAGE Homo sapiens
1.1.1.1 dimer 2 * 41000, class III isoenzyme chi ADH, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 0.245
-
 Pentanol
-
 Homo sapiens
1.1.1.1 0.467
-
 Vanillyl alcohol
-
 Homo sapiens
1.1.1.1 3.03
-
 12-hydroxydodecanoate
-
 Homo sapiens
1.1.1.1 3.33
-
 octanal
-
 Homo sapiens
1.1.1.1 7.33
-
 Octanol
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Homo sapiens
1.1.1.1 NADH
-
 Homo sapiens