BRENDA - Enzyme Database

Physical and enzymatic properties of a class II alcohol dehydrogenase isozyme of human liver: pi-ADH

Ditlow, C.C.; Holmquist, B.; Morelock, M.M.; Vallee, B.L.; Biochemistry 23, 6363-6368 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
1,10-phenanthroline
-
Homo sapiens
1.1.1.1
2,2'-bipyridine
-
Homo sapiens
1.1.1.1
4-Methylpyrazole
poor inhibitor, class II isoenzyme
Homo sapiens
1.1.1.1
EDTA
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.007
-
benzyl alcohol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.007
-
Octanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.032
-
3-Phenyl-1-propanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.034
-
Vanillyl alcohol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.06
-
16-hydroxyhexadecanoate
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.09
-
Pentanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.2
-
tryptophol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.23
-
12-hydroxydodecanoate
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
120
-
ethanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
210
-
Cyclohexanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
290
-
ethylene glycol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
310
-
2-deoxy-D-ribose
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
560
-
2-propanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
42000
-
x * 42000, SDS-PAGE
Homo sapiens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.1
Homo sapiens
-
class II isoenzyme: pi-ADH
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.1
class II isoenzyme: pi-ADH
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.1
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.1
1.47
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.1
4°C, pH 7.5, stable for 2-3 weeks
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.1
12-hydroxydodecanoate + NAD+
-
285569
Homo sapiens
12-oxododecanoic acid + NADH
-
-
-
?
1.1.1.1
16-hydroxyhexadecanoate + NAD+
-
285569
Homo sapiens
16-oxohexadecanoic acid + NADH
-
-
-
?
1.1.1.1
2-deoxy-D-ribose + NAD+
-
285569
Homo sapiens
? + NADH
-
-
-
?
1.1.1.1
3-phenyl-1-propanol + NAD+
-
285569
Homo sapiens
3-phenyl-1-propanone + NADH
-
-
-
?
1.1.1.1
benzyl alcohol + NAD+
-
285569
Homo sapiens
benzaldehyde + NADH
-
-
-
?
1.1.1.1
cyclohexanol + NAD+
-
285569
Homo sapiens
cyclohexanone + NADH
-
-
-
?
1.1.1.1
ethanol + NAD+
-
285569
Homo sapiens
acetaldehyde + NADH
-
-
-
?
1.1.1.1
ethylene glycol + NAD+
-
285569
Homo sapiens
? + NADH
-
-
-
?
1.1.1.1
methanol + NAD+
activity detected with class II isoenzyme pi-ADH
285569
Homo sapiens
formaldehyde + NADH + H+
-
-
-
?
1.1.1.1
octan-1-ol + NAD+
-
285569
Homo sapiens
n-octanal + NADH
-
-
-
?
1.1.1.1
pentanal + NAD+
-
285569
Homo sapiens
pentanone + NADH
-
-
-
?
1.1.1.1
propan-2-ol + NAD+
-
285569
Homo sapiens
acetone + NADH
-
-
-
?
1.1.1.1
tryptophol + NAD+
-
285569
Homo sapiens
? + NADH
-
-
-
?
1.1.1.1
vanillyl alcohol + NAD+
-
285569
Homo sapiens
vanillin + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.1
?
x * 42000, SDS-PAGE
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.583
-
Cyclohexanol
-
Homo sapiens
1.1.1.1
0.75
-
2-propanol
-
Homo sapiens
1.1.1.1
0.75
-
ethylene glycol
-
Homo sapiens
1.1.1.1
1.83
-
tryptophol
-
Homo sapiens
1.1.1.1
2.83
-
2-deoxy-D-ribose
-
Homo sapiens
1.1.1.1
4
-
12-hydroxydodecanoate
-
Homo sapiens
1.1.1.1
6.17
-
16-hydroxyhexadecanoate
-
Homo sapiens
1.1.1.1
7.5
-
3-Phenyl-1-propanol
-
Homo sapiens
1.1.1.1
7.83
-
ethanol
-
Homo sapiens
1.1.1.1
8
-
Pentanol
-
Homo sapiens
1.1.1.1
8.33
-
Octanol
-
Homo sapiens
1.1.1.1
8.67
-
Vanillyl alcohol
-
Homo sapiens
1.1.1.1
9.17
-
benzyl alcohol
-
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.1
10.4
-
-
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.1
8
12
about 30% of maximal activity at pH 8.0 and at pH 12.0
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Homo sapiens
1.1.1.1
NADH
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Homo sapiens
1.1.1.1
NADH
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
1,10-phenanthroline
-
Homo sapiens
1.1.1.1
2,2'-bipyridine
-
Homo sapiens
1.1.1.1
4-Methylpyrazole
poor inhibitor, class II isoenzyme
Homo sapiens
1.1.1.1
EDTA
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.007
-
benzyl alcohol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.007
-
Octanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.032
-
3-Phenyl-1-propanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.034
-
Vanillyl alcohol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.06
-
16-hydroxyhexadecanoate
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.09
-
Pentanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.2
-
tryptophol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
0.23
-
12-hydroxydodecanoate
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
120
-
ethanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
210
-
Cyclohexanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
290
-
ethylene glycol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
310
-
2-deoxy-D-ribose
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
1.1.1.1
560
-
2-propanol
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
42000
-
x * 42000, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.1
class II isoenzyme: pi-ADH
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.1
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.1
1.47
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.1
4°C, pH 7.5, stable for 2-3 weeks
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.1
12-hydroxydodecanoate + NAD+
-
285569
Homo sapiens
12-oxododecanoic acid + NADH
-
-
-
?
1.1.1.1
16-hydroxyhexadecanoate + NAD+
-
285569
Homo sapiens
16-oxohexadecanoic acid + NADH
-
-
-
?
1.1.1.1
2-deoxy-D-ribose + NAD+
-
285569
Homo sapiens
? + NADH
-
-
-
?
1.1.1.1
3-phenyl-1-propanol + NAD+
-
285569
Homo sapiens
3-phenyl-1-propanone + NADH
-
-
-
?
1.1.1.1
benzyl alcohol + NAD+
-
285569
Homo sapiens
benzaldehyde + NADH
-
-
-
?
1.1.1.1
cyclohexanol + NAD+
-
285569
Homo sapiens
cyclohexanone + NADH
-
-
-
?
1.1.1.1
ethanol + NAD+
-
285569
Homo sapiens
acetaldehyde + NADH
-
-
-
?
1.1.1.1
ethylene glycol + NAD+
-
285569
Homo sapiens
? + NADH
-
-
-
?
1.1.1.1
methanol + NAD+
activity detected with class II isoenzyme pi-ADH
285569
Homo sapiens
formaldehyde + NADH + H+
-
-
-
?
1.1.1.1
octan-1-ol + NAD+
-
285569
Homo sapiens
n-octanal + NADH
-
-
-
?
1.1.1.1
pentanal + NAD+
-
285569
Homo sapiens
pentanone + NADH
-
-
-
?
1.1.1.1
propan-2-ol + NAD+
-
285569
Homo sapiens
acetone + NADH
-
-
-
?
1.1.1.1
tryptophol + NAD+
-
285569
Homo sapiens
? + NADH
-
-
-
?
1.1.1.1
vanillyl alcohol + NAD+
-
285569
Homo sapiens
vanillin + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.1
?
x * 42000, SDS-PAGE
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.583
-
Cyclohexanol
-
Homo sapiens
1.1.1.1
0.75
-
2-propanol
-
Homo sapiens
1.1.1.1
0.75
-
ethylene glycol
-
Homo sapiens
1.1.1.1
1.83
-
tryptophol
-
Homo sapiens
1.1.1.1
2.83
-
2-deoxy-D-ribose
-
Homo sapiens
1.1.1.1
4
-
12-hydroxydodecanoate
-
Homo sapiens
1.1.1.1
6.17
-
16-hydroxyhexadecanoate
-
Homo sapiens
1.1.1.1
7.5
-
3-Phenyl-1-propanol
-
Homo sapiens
1.1.1.1
7.83
-
ethanol
-
Homo sapiens
1.1.1.1
8
-
Pentanol
-
Homo sapiens
1.1.1.1
8.33
-
Octanol
-
Homo sapiens
1.1.1.1
8.67
-
Vanillyl alcohol
-
Homo sapiens
1.1.1.1
9.17
-
benzyl alcohol
-
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.1
10.4
-
-
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.1
8
12
about 30% of maximal activity at pH 8.0 and at pH 12.0
Homo sapiens