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Literature summary extracted from

  • Johnson, H.A.; Pelletier, D.A.; Spormann, A.M.
    Isolation and characterisation of anaerobic ethylbenzene dehydrogenase, a novel Mo-Fe-S enzyme (2001), J. Bacteriol., 183, 4536-4542.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.99.2 in Escherichia coli DH5alpha Azoarcus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.99.2 membrane
-
 Azoarcus sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.99.2 iron sulfur cluster located in beta-subunit Azoarcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.99.2 23061
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
1.17.99.2 25000
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
1.17.99.2 35000
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
1.17.99.2 39643
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
1.17.99.2 70000
-
 gel filtration of purified enzyme Azoarcus sp.
1.17.99.2 100000
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
1.17.99.2 104226
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
1.17.99.2 160000
-
 calculated from DNA-sequence Azoarcus sp.
1.17.99.2 545000
-
 native PAGE Azoarcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.99.2 ethylbenzene + H2O + acceptor Azoarcus sp. anaerobic degradation of ethylbenzene in bacteria (S)-1-phenylethanol + reduced acceptor
-
 ?
1.17.99.2 ethylbenzene + H2O + acceptor Azoarcus sp. EB1 anaerobic degradation of ethylbenzene in bacteria (S)-1-phenylethanol + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.99.2 Azoarcus sp.
-
 EB1
-
1.17.99.2 Azoarcus sp. EB1
-
 EB1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.99.2
-
 Azoarcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.99.2 3-methyl-2-pentene + molybdopterin
-
 Azoarcus sp. ?
-
 ?
1.17.99.2 3-methyl-2-pentene + molybdopterin
-
 Azoarcus sp. EB1 ?
-
 ?
1.17.99.2 4-fluoro-ethylbenzene + molybdopterin
-
 Azoarcus sp. ?
-
 ?
1.17.99.2 4-fluoro-ethylbenzene + molybdopterin
-
 Azoarcus sp. EB1 ?
-
 ?
1.17.99.2 ethylbenzene + H2O + acceptor anaerobic degradation of ethylbenzene in bacteria Azoarcus sp. (S)-1-phenylethanol + reduced acceptor
-
 ?
1.17.99.2 ethylbenzene + H2O + acceptor anaerobic degradation of ethylbenzene in bacteria Azoarcus sp. EB1 (S)-1-phenylethanol + reduced acceptor
-
 ?
1.17.99.2 ethylbenzene + H2O + molybdopterin
-
 Azoarcus sp. (S)-1-phenylethanol + molybdopterin (reduced form)
-
 ?
1.17.99.2 ethylbenzene + H2O + molybdopterin
-
 Azoarcus sp. EB1 (S)-1-phenylethanol + molybdopterin (reduced form)
-
 ?
1.17.99.2 ethylidenecyclohexane + molybdopterin
-
 Azoarcus sp. ?
-
 ?
1.17.99.2 ethylidenecyclohexane + molybdopterin
-
 Azoarcus sp. EB1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.99.2 trimer alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.99.2 molybdopterin
-
 Azoarcus sp.