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Literature summary extracted from

  • Kniemeyer, O.; Heider, J.
    Ethylbenzene dehydrogenase, a novel hydrocarbon-oxidising molybdenum/iron-sulfur/heme enzyme (2001), J. Biol. Chem., 276, 21381-21386.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.99.2 O2 does not affect enzyme in cell extracts, inhibitory for purified enzyme Azoarcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.99.2 0.002
-
 ethylbenzene
-
 Azoarcus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.99.2 periplasm
-
 Azoarcus sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.99.2 [Fe(III) (C5H5)2]+
-
 Azoarcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.99.2 23000
-
 alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE Azoarcus sp.
1.17.99.2 43000
-
 alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE Azoarcus sp.
1.17.99.2 96000
-
 alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE Azoarcus sp.
1.17.99.2 155000
-
 gel filtration, native PAGE Azoarcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.99.2 ethylbenzene + H2O + acceptor Azoarcus sp. anaerobic degradation of ethylbenzene in bacteria (S)-1-phenylethanol + reduced acceptor
-
 ir
1.17.99.2 ethylbenzene + H2O + acceptor Azoarcus sp. EbN1 anaerobic degradation of ethylbenzene in bacteria (S)-1-phenylethanol + reduced acceptor
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.17.99.2 Azoarcus sp.
-
 EbN1
-
1.17.99.2 Azoarcus sp. EbN1
-
 EbN1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.99.2
-
 Azoarcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.99.2 0.0035
-
 activity towards n-propylbenzene of cell extract from cells grown on ethylbenzene Azoarcus sp.
1.17.99.2 0.0224
-
 activity towards ethylbenzene of cell extract from cells grown on ethylbenzene Azoarcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.99.2 ethylbenzene + H2O + acceptor anaerobic degradation of ethylbenzene in bacteria Azoarcus sp. (S)-1-phenylethanol + reduced acceptor
-
 ir
1.17.99.2 ethylbenzene + H2O + acceptor anaerobic degradation of ethylbenzene in bacteria Azoarcus sp. EbN1 (S)-1-phenylethanol + reduced acceptor
-
 ir
1.17.99.2 ethylbenzene + H2O + [Fe(III) (C5H5)2]+
-
 Azoarcus sp. (S)-1-phenylethanol + [Fe(II) (C5H5)2]
-
 ir
1.17.99.2 ethylbenzene + H2O + [Fe(III) (C5H5)2]+
-
 Azoarcus sp. EbN1 (S)-1-phenylethanol + [Fe(II) (C5H5)2]
-
 ir
1.17.99.2 n-propylbenzene + H2O + [Fe(III) (C5H5)2]+
-
 Azoarcus sp. (S)-1-phenylpropanol + [Fe(II) (C5H5)2]
-
 ?
1.17.99.2 n-propylbenzene + H2O + [Fe(III) (C5H5)2]+
-
 Azoarcus sp. EbN1 (S)-1-phenylpropanol + [Fe(II) (C5H5)2]
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.99.2 trimer alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE Azoarcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.99.2 7
-
-
 Azoarcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.99.2 5.5 9 about maximum activity at both pH Azoarcus sp.