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Literature summary extracted from

  • Horike, N.; Takemori, H.; Nonaka, Y.; Sonobe, H.; Okamoto, M.
    Molecular cloning of NADPH-cytochrome P-450 oxidoreductase from silkworm eggs (2000), Eur. J. Biochem., 267, 6914-6920.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.22 cDNA isolated from non-diapause eggs, expression in Escherichia coli BL21 as GST-fusion protein Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.22 77700
-
? * 77700, sequence determination Bombyx mori
1.14.99.22 103000
-
? * 103000, SDS-PAGE, recombinant GST-fusion protein Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.22 ecdysone + NADPH + O2 Bombyx mori
-
20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.22 Bombyx mori
-
silkworm
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.22 recombinant GST-fusion protein Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.99.22 additional information immunological analysis of enzyme content in diapause and non-diapause egg microsomes Bombyx mori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.22 27
-
recombinant GST-fusion protein, purified Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.22 alpha-ecdysone + NADPH + O2
-
Bombyx mori 20-hydroxyecdysone + NADP+ + H2O
-
?
1.14.99.22 ecdysone + NADPH + O2
-
Bombyx mori 20-hydroxyecdysone + NADP+ + H2O
-
?
1.14.99.22 additional information recombinant GST-fusion protein, expressed in E. coli, is also able to transfer electrons from NADPH to a microsomal steroid hydroxylase p-450 and support steroidogenesis Bombyx mori ?
-
?

Subunits

EC Number Subunits Comment Organism
1.14.99.22 ? ? * 77700, sequence determination Bombyx mori
1.14.99.22 ? ? * 103000, SDS-PAGE, recombinant GST-fusion protein Bombyx mori

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.22 cytochrome P450 essential Bombyx mori
1.14.99.22 NADPH
-
Bombyx mori