EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.99.22 | cDNA isolated from non-diapause eggs, expression in Escherichia coli BL21 as GST-fusion protein | Bombyx mori |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.99.22 | 77700 | - |
? * 77700, sequence determination | Bombyx mori |
1.14.99.22 | 103000 | - |
? * 103000, SDS-PAGE, recombinant GST-fusion protein | Bombyx mori |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.22 | ecdysone + NADPH + O2 | Bombyx mori | - |
20-hydroxyecdysone + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.99.22 | Bombyx mori | - |
silkworm | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.99.22 | recombinant GST-fusion protein | Bombyx mori |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.99.22 | additional information | immunological analysis of enzyme content in diapause and non-diapause egg microsomes | Bombyx mori | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.99.22 | 27 | - |
recombinant GST-fusion protein, purified | Bombyx mori |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.22 | alpha-ecdysone + NADPH + O2 | - |
Bombyx mori | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
1.14.99.22 | ecdysone + NADPH + O2 | - |
Bombyx mori | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
1.14.99.22 | additional information | recombinant GST-fusion protein, expressed in E. coli, is also able to transfer electrons from NADPH to a microsomal steroid hydroxylase p-450 and support steroidogenesis | Bombyx mori | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.99.22 | ? | ? * 77700, sequence determination | Bombyx mori |
1.14.99.22 | ? | ? * 103000, SDS-PAGE, recombinant GST-fusion protein | Bombyx mori |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.22 | cytochrome P450 | essential | Bombyx mori | |
1.14.99.22 | NADPH | - |
Bombyx mori |