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Literature summary extracted from
- Ecdysone 20-mono-oxygenase in the desert locust, Schistocerca gregaria (1984), Biochem. J., 223, 837-847.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.22 | ATP | increase of activity | Schistocerca gregaria |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.22 | 20-hydroxyecdysone | competitive inhibition | Schistocerca gregaria | |
| 1.14.99.22 | Ca2+ | - |
Schistocerca gregaria | |
| 1.14.99.22 | CO | partially reversible by irradiation with monochromatic light | Schistocerca gregaria | |
| 1.14.99.22 | EDTA | - |
Schistocerca gregaria | |
| 1.14.99.22 | Metyrapone | - |
Schistocerca gregaria | |
| 1.14.99.22 | Mg2+ | - |
Schistocerca gregaria | |
| 1.14.99.22 | Non-ionic detergents | Tween 20, Tween 80 and Triton X-100 | Schistocerca gregaria | |
| 1.14.99.22 | Tris/HCl-buffer | - |
Schistocerca gregaria | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.99.22 | 0.00071 | - |
ecdysone | - |
Schistocerca gregaria | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.99.22 | mitochondrion | membrane bound | Schistocerca gregaria | 5739 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.22 | ecdysone + NADPH + O2 | Schistocerca gregaria | serves as precursor of ecdysterone, the active moulting hormone in larval insects | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.22 | Schistocerca gregaria | - |
desert locust | - |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.99.22 | ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein | Schistocerca gregaria |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.99.22 | malpighian tubule | - |
Schistocerca gregaria | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.22 | 0.0000183 | - |
- |
Schistocerca gregaria |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.22 | alpha-ecdysone + NADPH + O2 | - |
Schistocerca gregaria | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
| 1.14.99.22 | ecdysone + NADPH + O2 | serves as precursor of ecdysterone, the active moulting hormone in larval insects | Schistocerca gregaria | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.22 | 35 | - |
- |
Schistocerca gregaria |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.22 | 6.8 | 8 | potassium phosphate buffer | Schistocerca gregaria |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.22 | cytochrome P450 | essential | Schistocerca gregaria | |
| 1.14.99.22 | NADH | increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor | Schistocerca gregaria | |
| 1.14.99.22 | NADPH | - |
Schistocerca gregaria | |
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