Data extracted from this reference:
Inhibitors
1.6.2.4
NADP+
Locusta migratoria migratorioides
1.14.99.22
20-hydroxyecdysone
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
7,8-Benzoflavone
Locusta migratoria migratorioides
1.14.99.22
CO
partially reversible by irradiation with monochromatic light
Locusta migratoria migratorioides
1.14.99.22
cytochrome c
Locusta migratoria migratorioides
1.14.99.22
ecdysterone
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
Metyrapone
Locusta migratoria migratorioides
1.14.99.22
NADP+
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
NEM
Locusta migratoria migratorioides
1.14.99.22
nicotinamide
Locusta migratoria migratorioides
1.14.99.22
oxidized cytochrome c
reversed by KCN
Locusta migratoria migratorioides
1.14.99.22
PCMB
Locusta migratoria migratorioides
1.14.99.22
Phospholipase A
Locusta migratoria migratorioides
1.14.99.22
Phospholipase C
Locusta migratoria migratorioides
1.14.99.22
Piperonylbutoxide
Locusta migratoria migratorioides
1.14.99.22
SKF 525A
Locusta migratoria migratorioides
KM Value [mM]
1.6.2.4
0.013
NADPH
Locusta migratoria migratorioides
1.14.99.22
0.00027
ecdysone
Locusta migratoria migratorioides
1.14.99.22
0.0105
NADPH
Locusta migratoria migratorioides
Localization
1.6.2.4
microsome
Locusta migratoria migratorioides
1.6.2.4
mitochondrion
Locusta migratoria migratorioides
5739
1.14.99.22
microsome
bound to sedimentable membrane
Locusta migratoria migratorioides
Natural Substrates/ Products (Substrates)
1.6.2.4
additional information
Locusta migratoria migratorioides
monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase
?
1.6.2.4
NADPH + ferricytochrome P450
Locusta migratoria migratorioides
during ecdysone hydroxylation
ferrocytochrome P450 + NADP+
?
1.14.99.22
ecdysone + NADPH + O2
Locusta migratoria migratorioides
serves as precursor of ecdysterone, the active moulting hormone in larval insects
20-hydroxyecdysone + NADP+ + H2O
Locusta migratoria migratorioides
?
Organism
1.6.2.4
Locusta migratoria migratorioides
1.14.99.22
Locusta migratoria migratorioides
L. migratoria migratorioides: african migration locust
Reaction
1.14.99.22
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O
NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein
Locusta migratoria migratorioides
Source Tissue
1.6.2.4
fat body
of larvae of both sexes
Locusta migratoria migratorioides
1.6.2.4
malpighian tubule
of larvae of both sexes
Locusta migratoria migratorioides
1.6.2.4
midgut
of larvae of both sexes
Locusta migratoria migratorioides
1.14.99.22
fat body
larval
Locusta migratoria migratorioides
1.14.99.22
malpighian tubule
Locusta migratoria migratorioides
Specific Activity [micromol/min/mg]
1.6.2.4
0.02
midgut and hindgut
Locusta migratoria migratorioides
1.6.2.4
0.029
malpighian tubules
Locusta migratoria migratorioides
1.6.2.4
0.051
fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000017
fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000041
mid- and hindgut
Locusta migratoria migratorioides
1.14.99.22
0.00000075
microsomes, fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000115
malphigian tubules
Locusta migratoria migratorioides
1.14.99.22
0.000003
microsomes, malphigian tubules
Locusta migratoria migratorioides
Storage Stability
1.14.99.22
-30°C, microsomal preparations with 30% v/v glycerol
Locusta migratoria migratorioides
Substrates and Products (Substrate)
1.6.2.4
2 ferricytochrome c + NADPH
additional electron acceptor: cytochrome P450
285452
Locusta migratoria migratorioides
2 ferrocytochrome c + NADP+ + H+
285452
Locusta migratoria migratorioides
?
1.6.2.4
additional information
hydroxylation of ecdysone
285452
Locusta migratoria migratorioides
?
?
1.6.2.4
additional information
monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase
285452
Locusta migratoria migratorioides
?
1.6.2.4
NADPH + ferricytochrome P450
during ecdysone hydroxylation
285452
Locusta migratoria migratorioides
ferrocytochrome P450 + NADP+
?
1.14.99.22
alpha-ecdysone + NADPH + O2
3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one, the 5alpha-epimer of ecdysone, 2beta-acetoxyecdysone, 3-hydroxyecdysone, 3-dehydro-ecdysone or ecdysterone are not hydroxylated
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
beta-ecdysone, ecdysterone
285452
Locusta migratoria migratorioides
?
1.14.99.22
ecdysone + NADPH + O2
serves as precursor of ecdysterone, the active moulting hormone in larval insects
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
285452
Locusta migratoria migratorioides
?
Temperature Optimum [°C]
1.14.99.22
40
Locusta migratoria migratorioides
pH Optimum
1.14.99.22
6.6
Locusta migratoria migratorioides
Cofactor
1.6.2.4
NADPH
Locusta migratoria migratorioides
1.14.99.22
cytochrome P450
essential
Locusta migratoria migratorioides
1.14.99.22
NADH
increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor
Locusta migratoria migratorioides
1.14.99.22
NADPH
NADP+ is not effective together with either malate, isocitrate, succinate, but with glucose-6-phosphate 25% of the activity is restored; ultimate hydrogen donor, cannot be substituted by NADH or ascorbate
Locusta migratoria migratorioides
Cofactor (protein specific)
1.6.2.4
NADPH
Locusta migratoria migratorioides
1.14.99.22
cytochrome P450
essential
Locusta migratoria migratorioides
1.14.99.22
NADH
increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor
Locusta migratoria migratorioides
1.14.99.22
NADPH
NADP+ is not effective together with either malate, isocitrate, succinate, but with glucose-6-phosphate 25% of the activity is restored; ultimate hydrogen donor, cannot be substituted by NADH or ascorbate
Locusta migratoria migratorioides
Inhibitors (protein specific)
1.6.2.4
NADP+
Locusta migratoria migratorioides
1.14.99.22
20-hydroxyecdysone
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
7,8-Benzoflavone
Locusta migratoria migratorioides
1.14.99.22
CO
partially reversible by irradiation with monochromatic light
Locusta migratoria migratorioides
1.14.99.22
cytochrome c
Locusta migratoria migratorioides
1.14.99.22
ecdysterone
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
Metyrapone
Locusta migratoria migratorioides
1.14.99.22
NADP+
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
NEM
Locusta migratoria migratorioides
1.14.99.22
nicotinamide
Locusta migratoria migratorioides
1.14.99.22
oxidized cytochrome c
reversed by KCN
Locusta migratoria migratorioides
1.14.99.22
PCMB
Locusta migratoria migratorioides
1.14.99.22
Phospholipase A
Locusta migratoria migratorioides
1.14.99.22
Phospholipase C
Locusta migratoria migratorioides
1.14.99.22
Piperonylbutoxide
Locusta migratoria migratorioides
1.14.99.22
SKF 525A
Locusta migratoria migratorioides
KM Value [mM] (protein specific)
1.6.2.4
0.013
NADPH
Locusta migratoria migratorioides
1.14.99.22
0.00027
ecdysone
Locusta migratoria migratorioides
1.14.99.22
0.0105
NADPH
Locusta migratoria migratorioides
Localization (protein specific)
1.6.2.4
microsome
Locusta migratoria migratorioides
1.6.2.4
mitochondrion
Locusta migratoria migratorioides
5739
1.14.99.22
microsome
bound to sedimentable membrane
Locusta migratoria migratorioides
Natural Substrates/ Products (Substrates) (protein specific)
1.6.2.4
additional information
Locusta migratoria migratorioides
monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase
?
1.6.2.4
NADPH + ferricytochrome P450
Locusta migratoria migratorioides
during ecdysone hydroxylation
ferrocytochrome P450 + NADP+
?
1.14.99.22
ecdysone + NADPH + O2
Locusta migratoria migratorioides
serves as precursor of ecdysterone, the active moulting hormone in larval insects
20-hydroxyecdysone + NADP+ + H2O
Locusta migratoria migratorioides
?
Source Tissue (protein specific)
1.6.2.4
fat body
of larvae of both sexes
Locusta migratoria migratorioides
1.6.2.4
malpighian tubule
of larvae of both sexes
Locusta migratoria migratorioides
1.6.2.4
midgut
of larvae of both sexes
Locusta migratoria migratorioides
1.14.99.22
fat body
larval
Locusta migratoria migratorioides
1.14.99.22
malpighian tubule
Locusta migratoria migratorioides
Specific Activity [micromol/min/mg] (protein specific)
1.6.2.4
0.02
midgut and hindgut
Locusta migratoria migratorioides
1.6.2.4
0.029
malpighian tubules
Locusta migratoria migratorioides
1.6.2.4
0.051
fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000017
fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000041
mid- and hindgut
Locusta migratoria migratorioides
1.14.99.22
0.00000075
microsomes, fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000115
malphigian tubules
Locusta migratoria migratorioides
1.14.99.22
0.000003
microsomes, malphigian tubules
Locusta migratoria migratorioides
Storage Stability (protein specific)
1.14.99.22
-30°C, microsomal preparations with 30% v/v glycerol
Locusta migratoria migratorioides
Substrates and Products (Substrate) (protein specific)
1.6.2.4
2 ferricytochrome c + NADPH
additional electron acceptor: cytochrome P450
285452
Locusta migratoria migratorioides
2 ferrocytochrome c + NADP+ + H+
285452
Locusta migratoria migratorioides
?
1.6.2.4
additional information
hydroxylation of ecdysone
285452
Locusta migratoria migratorioides
?
?
1.6.2.4
additional information
monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase
285452
Locusta migratoria migratorioides
?
1.6.2.4
NADPH + ferricytochrome P450
during ecdysone hydroxylation
285452
Locusta migratoria migratorioides
ferrocytochrome P450 + NADP+
?
1.14.99.22
alpha-ecdysone + NADPH + O2
3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one, the 5alpha-epimer of ecdysone, 2beta-acetoxyecdysone, 3-hydroxyecdysone, 3-dehydro-ecdysone or ecdysterone are not hydroxylated
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
beta-ecdysone, ecdysterone
285452
Locusta migratoria migratorioides
?
1.14.99.22
ecdysone + NADPH + O2
serves as precursor of ecdysterone, the active moulting hormone in larval insects
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
285452
Locusta migratoria migratorioides
?
Temperature Optimum [°C] (protein specific)
1.14.99.22
40
Locusta migratoria migratorioides
pH Optimum (protein specific)
1.14.99.22
6.6
Locusta migratoria migratorioides