EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.4 | NADP+ | - |
Locusta migratoria migratorioides | |
1.14.99.22 | 20-hydroxyecdysone | competitive inhibition | Locusta migratoria migratorioides | |
1.14.99.22 | 7,8-Benzoflavone | - |
Locusta migratoria migratorioides | |
1.14.99.22 | CO | partially reversible by irradiation with monochromatic light | Locusta migratoria migratorioides | |
1.14.99.22 | cytochrome c | - |
Locusta migratoria migratorioides | |
1.14.99.22 | ecdysterone | competitive inhibition | Locusta migratoria migratorioides | |
1.14.99.22 | Metyrapone | - |
Locusta migratoria migratorioides | |
1.14.99.22 | NADP+ | competitive inhibition | Locusta migratoria migratorioides | |
1.14.99.22 | NEM | - |
Locusta migratoria migratorioides | |
1.14.99.22 | nicotinamide | - |
Locusta migratoria migratorioides | |
1.14.99.22 | oxidized cytochrome c | reversed by KCN | Locusta migratoria migratorioides | |
1.14.99.22 | PCMB | - |
Locusta migratoria migratorioides | |
1.14.99.22 | Phospholipase A | - |
Locusta migratoria migratorioides | |
1.14.99.22 | Phospholipase C | - |
Locusta migratoria migratorioides | |
1.14.99.22 | Piperonylbutoxide | - |
Locusta migratoria migratorioides | |
1.14.99.22 | SKF 525A | - |
Locusta migratoria migratorioides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.4 | 0.013 | - |
NADPH | - |
Locusta migratoria migratorioides | |
1.14.99.22 | 0.00027 | - |
ecdysone | - |
Locusta migratoria migratorioides | |
1.14.99.22 | 0.0105 | - |
NADPH | - |
Locusta migratoria migratorioides |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.6.2.4 | microsome | - |
Locusta migratoria migratorioides | - |
- |
1.6.2.4 | mitochondrion | - |
Locusta migratoria migratorioides | 5739 | - |
1.14.99.22 | microsome | bound to sedimentable membrane | Locusta migratoria migratorioides | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.4 | additional information | Locusta migratoria migratorioides | monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase | ? | - |
? | |
1.6.2.4 | NADPH + ferricytochrome P450 | Locusta migratoria migratorioides | during ecdysone hydroxylation | ferrocytochrome P450 + NADP+ | - |
? | |
1.14.99.22 | ecdysone + NADPH + O2 | Locusta migratoria migratorioides | serves as precursor of ecdysterone, the active moulting hormone in larval insects | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.2.4 | Locusta migratoria migratorioides | - |
- |
- |
1.14.99.22 | Locusta migratoria migratorioides | - |
L. migratoria migratorioides: african migration locust | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.99.22 | ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein | Locusta migratoria migratorioides |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.6.2.4 | fat body | of larvae of both sexes | Locusta migratoria migratorioides | - |
1.6.2.4 | malpighian tubule | of larvae of both sexes | Locusta migratoria migratorioides | - |
1.6.2.4 | midgut | of larvae of both sexes | Locusta migratoria migratorioides | - |
1.14.99.22 | fat body | larval | Locusta migratoria migratorioides | - |
1.14.99.22 | malpighian tubule | - |
Locusta migratoria migratorioides | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.6.2.4 | 0.02 | - |
midgut and hindgut | Locusta migratoria migratorioides |
1.6.2.4 | 0.029 | - |
malpighian tubules | Locusta migratoria migratorioides |
1.6.2.4 | 0.051 | - |
fat body | Locusta migratoria migratorioides |
1.14.99.22 | 0.00000017 | - |
fat body | Locusta migratoria migratorioides |
1.14.99.22 | 0.00000041 | - |
mid- and hindgut | Locusta migratoria migratorioides |
1.14.99.22 | 0.00000075 | - |
microsomes, fat body | Locusta migratoria migratorioides |
1.14.99.22 | 0.00000115 | - |
malphigian tubules | Locusta migratoria migratorioides |
1.14.99.22 | 0.000003 | - |
microsomes, malphigian tubules | Locusta migratoria migratorioides |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.99.22 | -30°C, microsomal preparations with 30% v/v glycerol | Locusta migratoria migratorioides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: cytochrome P450 | Locusta migratoria migratorioides | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
1.6.2.4 | additional information | hydroxylation of ecdysone | Locusta migratoria migratorioides | ? | - |
? | |
1.6.2.4 | additional information | monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase | Locusta migratoria migratorioides | ? | - |
? | |
1.6.2.4 | NADPH + ferricytochrome P450 | during ecdysone hydroxylation | Locusta migratoria migratorioides | ferrocytochrome P450 + NADP+ | - |
? | |
1.14.99.22 | alpha-ecdysone + NADPH + O2 | 3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one, the 5alpha-epimer of ecdysone, 2beta-acetoxyecdysone, 3-hydroxyecdysone, 3-dehydro-ecdysone or ecdysterone are not hydroxylated | Locusta migratoria migratorioides | 20-hydroxyecdysone + NADP+ + H2O | beta-ecdysone, ecdysterone | ? | |
1.14.99.22 | ecdysone + NADPH + O2 | serves as precursor of ecdysterone, the active moulting hormone in larval insects | Locusta migratoria migratorioides | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.99.22 | 40 | - |
- |
Locusta migratoria migratorioides |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.99.22 | 6.6 | - |
- |
Locusta migratoria migratorioides |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.4 | NADPH | - |
Locusta migratoria migratorioides | |
1.14.99.22 | cytochrome P450 | essential | Locusta migratoria migratorioides | |
1.14.99.22 | NADH | increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor | Locusta migratoria migratorioides | |
1.14.99.22 | NADPH | ultimate hydrogen donor, cannot be substituted by NADH or ascorbate | Locusta migratoria migratorioides | |
1.14.99.22 | NADPH | NADP+ is not effective together with either malate, isocitrate, succinate, but with glucose-6-phosphate 25% of the activity is restored | Locusta migratoria migratorioides |