Ecdysterone biosynthesis: a microsomal cytochrome-P-450-linked ecdysone 20-monooxygenase from tissues of the African migratory locust
Feyereisen, R.; Durst, F.; Eur. J. Biochem. 88, 37-47 (1978) 
Data extracted from this reference:
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.6.2.4
NADP+
-
Locusta migratoria migratorioides
1.14.99.22
20-hydroxyecdysone
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
7,8-Benzoflavone
-
Locusta migratoria migratorioides
1.14.99.22
CO
partially reversible by irradiation with monochromatic light
Locusta migratoria migratorioides
1.14.99.22
cytochrome c
-
Locusta migratoria migratorioides
1.14.99.22
ecdysterone
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
Metyrapone
-
Locusta migratoria migratorioides
1.14.99.22
NADP+
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
NEM
-
Locusta migratoria migratorioides
1.14.99.22
nicotinamide
-
Locusta migratoria migratorioides
1.14.99.22
oxidized cytochrome c
reversed by KCN
Locusta migratoria migratorioides
1.14.99.22
PCMB
-
Locusta migratoria migratorioides
1.14.99.22
Phospholipase A
-
Locusta migratoria migratorioides
1.14.99.22
Phospholipase C
-
Locusta migratoria migratorioides
1.14.99.22
Piperonylbutoxide
-
Locusta migratoria migratorioides
1.14.99.22
SKF 525A
-
Locusta migratoria migratorioides
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6.2.4
0.013
-
NADPH
-
Locusta migratoria migratorioides
1.14.99.22
0.00027
-
ecdysone
-
Locusta migratoria migratorioides
1.14.99.22
0.0105
-
NADPH
-
Locusta migratoria migratorioides
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.6.2.4
microsome
-
Locusta migratoria migratorioides
-
-
1.6.2.4
mitochondrion
-
Locusta migratoria migratorioides
5739
-
1.14.99.22
microsome
bound to sedimentable membrane
Locusta migratoria migratorioides
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.6.2.4
additional information
Locusta migratoria migratorioides
monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase
?
-
-
-
1.6.2.4
NADPH + ferricytochrome P450
Locusta migratoria migratorioides
during ecdysone hydroxylation
ferrocytochrome P450 + NADP+
-
-
?
1.14.99.22
ecdysone + NADPH + O2
Locusta migratoria migratorioides
serves as precursor of ecdysterone, the active moulting hormone in larval insects
20-hydroxyecdysone + NADP+ + H2O
-
Locusta migratoria migratorioides
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.6.2.4
Locusta migratoria migratorioides
-
-
-
1.14.99.22
Locusta migratoria migratorioides
-
L. migratoria migratorioides: african migration locust
-
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
1.14.99.22
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O
NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein
Locusta migratoria migratorioides
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.6.2.4
fat body
of larvae of both sexes
Locusta migratoria migratorioides
-
1.6.2.4
malpighian tubule
of larvae of both sexes
Locusta migratoria migratorioides
-
1.6.2.4
midgut
of larvae of both sexes
Locusta migratoria migratorioides
-
1.14.99.22
fat body
larval
Locusta migratoria migratorioides
-
1.14.99.22
malpighian tubule
-
Locusta migratoria migratorioides
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.6.2.4
0.02
-
midgut and hindgut
Locusta migratoria migratorioides
1.6.2.4
0.029
-
malpighian tubules
Locusta migratoria migratorioides
1.6.2.4
0.051
-
fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000017
-
fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000041
-
mid- and hindgut
Locusta migratoria migratorioides
1.14.99.22
0.00000075
-
microsomes, fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000115
-
malphigian tubules
Locusta migratoria migratorioides
1.14.99.22
0.000003
-
microsomes, malphigian tubules
Locusta migratoria migratorioides
Storage Stability
EC Number
Storage Stability
Organism
1.14.99.22
-30°C, microsomal preparations with 30% v/v glycerol
Locusta migratoria migratorioides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.6.2.4
2 ferricytochrome c + NADPH
additional electron acceptor: cytochrome P450
285452
Locusta migratoria migratorioides
2 ferrocytochrome c + NADP+ + H+
-
285452
Locusta migratoria migratorioides
?
1.6.2.4
additional information
hydroxylation of ecdysone
285452
Locusta migratoria migratorioides
?
-
-
-
?
1.6.2.4
additional information
monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase
285452
Locusta migratoria migratorioides
?
-
-
-
-
1.6.2.4
NADPH + ferricytochrome P450
during ecdysone hydroxylation
285452
Locusta migratoria migratorioides
ferrocytochrome P450 + NADP+
-
-
-
?
1.14.99.22
alpha-ecdysone + NADPH + O2
3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one, the 5alpha-epimer of ecdysone, 2beta-acetoxyecdysone, 3-hydroxyecdysone, 3-dehydro-ecdysone or ecdysterone are not hydroxylated
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
beta-ecdysone, ecdysterone
285452
Locusta migratoria migratorioides
?
1.14.99.22
ecdysone + NADPH + O2
serves as precursor of ecdysterone, the active moulting hormone in larval insects
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
-
285452
Locusta migratoria migratorioides
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.99.22
40
-
-
Locusta migratoria migratorioides
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.99.22
6.6
-
-
Locusta migratoria migratorioides
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.6.2.4
NADPH
-
Locusta migratoria migratorioides
1.14.99.22
cytochrome P450
essential
Locusta migratoria migratorioides
1.14.99.22
NADH
increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor
Locusta migratoria migratorioides
1.14.99.22
NADPH
NADP+ is not effective together with either malate, isocitrate, succinate, but with glucose-6-phosphate 25% of the activity is restored; ultimate hydrogen donor, cannot be substituted by NADH or ascorbate
Locusta migratoria migratorioides
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.6.2.4
NADPH
-
Locusta migratoria migratorioides
1.14.99.22
cytochrome P450
essential
Locusta migratoria migratorioides
1.14.99.22
NADH
increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor
Locusta migratoria migratorioides
1.14.99.22
NADPH
NADP+ is not effective together with either malate, isocitrate, succinate, but with glucose-6-phosphate 25% of the activity is restored; ultimate hydrogen donor, cannot be substituted by NADH or ascorbate
Locusta migratoria migratorioides
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.6.2.4
NADP+
-
Locusta migratoria migratorioides
1.14.99.22
20-hydroxyecdysone
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
7,8-Benzoflavone
-
Locusta migratoria migratorioides
1.14.99.22
CO
partially reversible by irradiation with monochromatic light
Locusta migratoria migratorioides
1.14.99.22
cytochrome c
-
Locusta migratoria migratorioides
1.14.99.22
ecdysterone
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
Metyrapone
-
Locusta migratoria migratorioides
1.14.99.22
NADP+
competitive inhibition
Locusta migratoria migratorioides
1.14.99.22
NEM
-
Locusta migratoria migratorioides
1.14.99.22
nicotinamide
-
Locusta migratoria migratorioides
1.14.99.22
oxidized cytochrome c
reversed by KCN
Locusta migratoria migratorioides
1.14.99.22
PCMB
-
Locusta migratoria migratorioides
1.14.99.22
Phospholipase A
-
Locusta migratoria migratorioides
1.14.99.22
Phospholipase C
-
Locusta migratoria migratorioides
1.14.99.22
Piperonylbutoxide
-
Locusta migratoria migratorioides
1.14.99.22
SKF 525A
-
Locusta migratoria migratorioides
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6.2.4
0.013
-
NADPH
-
Locusta migratoria migratorioides
1.14.99.22
0.00027
-
ecdysone
-
Locusta migratoria migratorioides
1.14.99.22
0.0105
-
NADPH
-
Locusta migratoria migratorioides
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.6.2.4
microsome
-
Locusta migratoria migratorioides
-
-
1.6.2.4
mitochondrion
-
Locusta migratoria migratorioides
5739
-
1.14.99.22
microsome
bound to sedimentable membrane
Locusta migratoria migratorioides
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.6.2.4
additional information
Locusta migratoria migratorioides
monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase
?
-
-
-
1.6.2.4
NADPH + ferricytochrome P450
Locusta migratoria migratorioides
during ecdysone hydroxylation
ferrocytochrome P450 + NADP+
-
-
?
1.14.99.22
ecdysone + NADPH + O2
Locusta migratoria migratorioides
serves as precursor of ecdysterone, the active moulting hormone in larval insects
20-hydroxyecdysone + NADP+ + H2O
-
Locusta migratoria migratorioides
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.6.2.4
fat body
of larvae of both sexes
Locusta migratoria migratorioides
-
1.6.2.4
malpighian tubule
of larvae of both sexes
Locusta migratoria migratorioides
-
1.6.2.4
midgut
of larvae of both sexes
Locusta migratoria migratorioides
-
1.14.99.22
fat body
larval
Locusta migratoria migratorioides
-
1.14.99.22
malpighian tubule
-
Locusta migratoria migratorioides
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.6.2.4
0.02
-
midgut and hindgut
Locusta migratoria migratorioides
1.6.2.4
0.029
-
malpighian tubules
Locusta migratoria migratorioides
1.6.2.4
0.051
-
fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000017
-
fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000041
-
mid- and hindgut
Locusta migratoria migratorioides
1.14.99.22
0.00000075
-
microsomes, fat body
Locusta migratoria migratorioides
1.14.99.22
0.00000115
-
malphigian tubules
Locusta migratoria migratorioides
1.14.99.22
0.000003
-
microsomes, malphigian tubules
Locusta migratoria migratorioides
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.99.22
-30°C, microsomal preparations with 30% v/v glycerol
Locusta migratoria migratorioides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.6.2.4
2 ferricytochrome c + NADPH
additional electron acceptor: cytochrome P450
285452
Locusta migratoria migratorioides
2 ferrocytochrome c + NADP+ + H+
-
285452
Locusta migratoria migratorioides
?
1.6.2.4
additional information
hydroxylation of ecdysone
285452
Locusta migratoria migratorioides
?
-
-
-
?
1.6.2.4
additional information
monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase
285452
Locusta migratoria migratorioides
?
-
-
-
-
1.6.2.4
NADPH + ferricytochrome P450
during ecdysone hydroxylation
285452
Locusta migratoria migratorioides
ferrocytochrome P450 + NADP+
-
-
-
?
1.14.99.22
alpha-ecdysone + NADPH + O2
3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one, the 5alpha-epimer of ecdysone, 2beta-acetoxyecdysone, 3-hydroxyecdysone, 3-dehydro-ecdysone or ecdysterone are not hydroxylated
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
beta-ecdysone, ecdysterone
285452
Locusta migratoria migratorioides
?
1.14.99.22
ecdysone + NADPH + O2
serves as precursor of ecdysterone, the active moulting hormone in larval insects
285452
Locusta migratoria migratorioides
20-hydroxyecdysone + NADP+ + H2O
-
285452
Locusta migratoria migratorioides
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.99.22
40
-
-
Locusta migratoria migratorioides
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.99.22
6.6
-
-
Locusta migratoria migratorioides