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Literature summary extracted from

  • Feyereisen, R.; Durst, F.
    Ecdysterone biosynthesis: a microsomal cytochrome-P-450-linked ecdysone 20-monooxygenase from tissues of the African migratory locust (1978), Eur. J. Biochem., 88, 37-47.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.4 NADP+
-
Locusta migratoria migratorioides
1.14.99.22 20-hydroxyecdysone competitive inhibition Locusta migratoria migratorioides
1.14.99.22 7,8-Benzoflavone
-
Locusta migratoria migratorioides
1.14.99.22 CO partially reversible by irradiation with monochromatic light Locusta migratoria migratorioides
1.14.99.22 cytochrome c
-
Locusta migratoria migratorioides
1.14.99.22 ecdysterone competitive inhibition Locusta migratoria migratorioides
1.14.99.22 Metyrapone
-
Locusta migratoria migratorioides
1.14.99.22 NADP+ competitive inhibition Locusta migratoria migratorioides
1.14.99.22 NEM
-
Locusta migratoria migratorioides
1.14.99.22 nicotinamide
-
Locusta migratoria migratorioides
1.14.99.22 oxidized cytochrome c reversed by KCN Locusta migratoria migratorioides
1.14.99.22 PCMB
-
Locusta migratoria migratorioides
1.14.99.22 Phospholipase A
-
Locusta migratoria migratorioides
1.14.99.22 Phospholipase C
-
Locusta migratoria migratorioides
1.14.99.22 Piperonylbutoxide
-
Locusta migratoria migratorioides
1.14.99.22 SKF 525A
-
Locusta migratoria migratorioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.013
-
NADPH
-
Locusta migratoria migratorioides
1.14.99.22 0.00027
-
ecdysone
-
Locusta migratoria migratorioides
1.14.99.22 0.0105
-
NADPH
-
Locusta migratoria migratorioides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 microsome
-
Locusta migratoria migratorioides
-
-
1.6.2.4 mitochondrion
-
Locusta migratoria migratorioides 5739
-
1.14.99.22 microsome bound to sedimentable membrane Locusta migratoria migratorioides
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 additional information Locusta migratoria migratorioides monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase ?
-
?
1.6.2.4 NADPH + ferricytochrome P450 Locusta migratoria migratorioides during ecdysone hydroxylation ferrocytochrome P450 + NADP+
-
?
1.14.99.22 ecdysone + NADPH + O2 Locusta migratoria migratorioides serves as precursor of ecdysterone, the active moulting hormone in larval insects 20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Locusta migratoria migratorioides
-
-
-
1.14.99.22 Locusta migratoria migratorioides
-
L. migratoria migratorioides: african migration locust
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.99.22 ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein Locusta migratoria migratorioides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 fat body of larvae of both sexes Locusta migratoria migratorioides
-
1.6.2.4 malpighian tubule of larvae of both sexes Locusta migratoria migratorioides
-
1.6.2.4 midgut of larvae of both sexes Locusta migratoria migratorioides
-
1.14.99.22 fat body larval Locusta migratoria migratorioides
-
1.14.99.22 malpighian tubule
-
Locusta migratoria migratorioides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 0.02
-
midgut and hindgut Locusta migratoria migratorioides
1.6.2.4 0.029
-
malpighian tubules Locusta migratoria migratorioides
1.6.2.4 0.051
-
fat body Locusta migratoria migratorioides
1.14.99.22 0.00000017
-
fat body Locusta migratoria migratorioides
1.14.99.22 0.00000041
-
mid- and hindgut Locusta migratoria migratorioides
1.14.99.22 0.00000075
-
microsomes, fat body Locusta migratoria migratorioides
1.14.99.22 0.00000115
-
malphigian tubules Locusta migratoria migratorioides
1.14.99.22 0.000003
-
microsomes, malphigian tubules Locusta migratoria migratorioides

Storage Stability

EC Number Storage Stability Organism
1.14.99.22 -30°C, microsomal preparations with 30% v/v glycerol Locusta migratoria migratorioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: cytochrome P450 Locusta migratoria migratorioides 2 ferrocytochrome c + NADP+ + H+
-
?
1.6.2.4 additional information hydroxylation of ecdysone Locusta migratoria migratorioides ?
-
?
1.6.2.4 additional information monooxygenase system composed of ecdysone 20-monooxygenase, cytochrome P-450 and NADPH-cytochrome c reductase Locusta migratoria migratorioides ?
-
?
1.6.2.4 NADPH + ferricytochrome P450 during ecdysone hydroxylation Locusta migratoria migratorioides ferrocytochrome P450 + NADP+
-
?
1.14.99.22 alpha-ecdysone + NADPH + O2 3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one, the 5alpha-epimer of ecdysone, 2beta-acetoxyecdysone, 3-hydroxyecdysone, 3-dehydro-ecdysone or ecdysterone are not hydroxylated Locusta migratoria migratorioides 20-hydroxyecdysone + NADP+ + H2O beta-ecdysone, ecdysterone ?
1.14.99.22 ecdysone + NADPH + O2 serves as precursor of ecdysterone, the active moulting hormone in larval insects Locusta migratoria migratorioides 20-hydroxyecdysone + NADP+ + H2O
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.22 40
-
-
Locusta migratoria migratorioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.22 6.6
-
-
Locusta migratoria migratorioides

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 NADPH
-
Locusta migratoria migratorioides
1.14.99.22 cytochrome P450 essential Locusta migratoria migratorioides
1.14.99.22 NADH increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor Locusta migratoria migratorioides
1.14.99.22 NADPH ultimate hydrogen donor, cannot be substituted by NADH or ascorbate Locusta migratoria migratorioides
1.14.99.22 NADPH NADP+ is not effective together with either malate, isocitrate, succinate, but with glucose-6-phosphate 25% of the activity is restored Locusta migratoria migratorioides