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Literature summary extracted from

  • Hiwatashi, A.; Ichikawa, Y.
    Purification and reconstitution of the steroid 21-hydroxylase system (cytochrome P-450-linked mixed function oxidase system) of bovine adrenocortical microsomes (1981), Biochim. Biophys. Acta, 664, 33-48.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.16 Emulgen 911
-
 Bos taurus
1.14.14.16 Emulgen 913 maximum with 0.008% v/v Bos taurus
1.14.14.16 lysophosphatidylcholine
-
 Bos taurus
1.14.14.16 sodium cholate
-
 Bos taurus
1.14.14.16 sodium deoxycholate
-
 Bos taurus
1.14.14.16 Triton X-100
-
 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.16 antibody to cytochrome P-450BPA
-
 Bos taurus
1.14.14.16 antibody to NADPH-cytochrome P-450 reductase
-
 Bos taurus
1.14.14.16 carbon monoxide
-
 Bos taurus
1.14.14.16 additional information not inhibited by catalase; not inhibited by superoxide dismutase, cytochrome b5, NADH-cytochrome b5 reductase Bos taurus
1.14.14.16 Phenylisocyanide inhibition at 0.5 mM Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.16 microsome
-
 Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.16 47500
-
 SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.16 Bos taurus
-
 beef
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.14.16 glycoprotein 3.6% carbohydrate Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.16 enzyme system consisting of cytochrome P-450S21 and NADPH-cytochrome P-450 reductase Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.16 adrenal gland
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.16 0.0052
-
 N-demethylation of (+)-benzphetamine Bos taurus
1.14.14.16 0.0077
-
 21-hydroxylation of progesterone Bos taurus
1.14.14.16 0.0195
-
 cytochrome P-450-linked mixed function oxidase system from adrenal gland, 21-hydroxylation of 17alpha-hydroxyprogesterone Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.14.14.16 -70°C, 0.2 M potassium phospate, pH 7.4, 20% glycerol, 0.1 mM EDTA, 3 months without loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.16 (+)-benzphetamine + [reduced NADPH-cytochrome P-450 reductase] + O2
-
 Bos taurus N-demethyl-benzphetamine + [oxidized NADPH-cytochrome P-450 reductase] + H2O
-
 ?
1.14.14.16 17alpha-hydroxyprogesterone + NADH + O2
-
 Bos taurus 17,21-dihydroxyprogesterone + NAD+ + H2O
-
 ?
1.14.14.16 DELTA5-pregnen-3beta-ol-20-one + NADH + O2 no substrate of cytochrome P-450-linked mixed function oxidase system Bos taurus deoxycorticosterone + NAD+ + H2O
-
 ?
1.14.14.16 progesterone + NADH + O2
-
 Bos taurus deoxycorticosterone + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.16 More steroid 21-hydroxylase system consists of cytochrome P-450S21, NADPH-cytochrome P-450 reductase, EC 1.6.2.4, and steroid 21-monooxygenase, EC 1.14.99.10 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.16 7.4
-
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.16 cytochrome P450S21 steroid 21-hydroxylase system consists of cytochrome P-450S21, NADPH-cytochrome P-450 reductase (EC 1.6.2.4) and steroid 21-monooxygenase (EC 1.14.99.10) Bos taurus