EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.16 | antimycin A | 15% inhibition at 1mg per l | Bos taurus | |
1.14.14.16 | ascorbate | 10% inhibition at 10 mM | Bos taurus | |
1.14.14.16 | azide | 9% inhibition at 1 mM | Bos taurus | |
1.14.14.16 | carbon monoxide | dark 65% inhibition at 90%, light 57% inhibition at 90% | Bos taurus | |
1.14.14.16 | CuSO4 | 50% inhibition at 1 mM | Bos taurus | |
1.14.14.16 | cyanide | 14% inhibition at 1 mM | Bos taurus | |
1.14.14.16 | cytochrome c | complete inhibition at 0.1 mM, 0.003 mM | Bos taurus | |
1.14.14.16 | diethylaminoethyldiphenylpropylacetic acid SKF 525 A | 9% inhibition at 1 mM | Bos taurus | |
1.14.14.16 | HgCl2 | complete inhibition at 0.1 mM | Bos taurus | |
1.14.14.16 | additional information | not inhibited by catalase; not inhibited by iodoacetate, o-iodosobenzoate, glutathione, EDTA, dipyridyl, quinacrine, ribonuclease, cytochrome c + CN-; not inhibited by metal ions such as Cr3+, Fe3+, Zn2+, Pb2+, Mn2+, Co2+ at 1 mM | Bos taurus | |
1.14.14.16 | p-chloromercuribenzoate | complete inhibition at 1 mM, but not inhibitory at 0.1 mM | Bos taurus | |
1.14.14.16 | sodium o-(3-hydroxymercuri-2-methoxypropyl)carbamyl-phenoxyacetc acid (mersalyl) | complete inhibition at 1 mM | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.16 | microsome | - |
Bos taurus | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.16 | a steroid + electron donor + O2 | Bos taurus | essential step in synthesis of steroid hormones by adrenal gland | a 21-hydroxysteroid + oxidized electron donor + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.16 | Bos taurus | - |
beef | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.16 | adrenal gland | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.16 | 11,17-dihydroxyprogesterone + NADH + O2 | - |
Bos taurus | cortisol + NAD+ + H2O | - |
? | |
1.14.14.16 | 11beta-hydroxyprogesterone + NADH + O2 | - |
Bos taurus | corticosterone + NAD+ + H2O | - |
? | |
1.14.14.16 | 17alpha-hydroxyprogesterone + NADH + O2 | - |
Bos taurus | 17,21-dihydroxyprogesterone + NAD+ + H2O | - |
? | |
1.14.14.16 | a steroid + electron donor + O2 | essential step in synthesis of steroid hormones by adrenal gland | Bos taurus | a 21-hydroxysteroid + oxidized electron donor + H2O | - |
? | |
1.14.14.16 | DELTA5-pregnen-3beta,17alpha-diol-20-one + NADH + O2 | - |
Bos taurus | ? + NAD+ + H2O | - |
? | |
1.14.14.16 | DELTA5-pregnen-3beta-ol-20-one + NADH + O2 | - |
Bos taurus | deoxycorticosterone + NAD+ + H2O | very small yields, presumably via dehydrogenation followed by C-21 hydroxylation | ? | |
1.14.14.16 | progesterone + NADH + O2 | - |
Bos taurus | deoxycorticosterone + NAD+ + H2O | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.16 | 6.5 | 7 | - |
Bos taurus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.16 | 5.5 | 8 | about 50% of activity maximum at pH 5.5 and 8.0 | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.16 | NADH | NADH is 50% as effective as NADPH | Bos taurus | |
1.14.14.16 | NADPH | - |
Bos taurus |