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Literature summary extracted from

  • Kashiwagi, K.; Dafeldecker, W.P.; Salhanick, H.A.
    Purification and characterization of mitochondrial cytochrome P-450 associated with cholesterol side chain cleavage from bovine corpus luteum (1980), J. Biol. Chem., 255, 2606-2611.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 mitochondrial inner membrane
-
 Bos taurus 5743
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.6 46000
-
 2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria Bos taurus
1.14.15.6 48000
-
 2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria Bos taurus
1.14.15.6 53000
-
 2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria Bos taurus
1.14.15.6 60000
-
 2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria Bos taurus
1.14.15.6 97000
-
 sedimentation equilibrium Bos taurus
1.14.15.6 100000
-
 native-PAGE, incomplete enzyme or molecular environmental conditions not optimal Bos taurus
1.14.15.6 200000
-
 native-PAGE, lowest single unit with high enzymatic activity Bos taurus
1.14.15.6 220000
-
 corpus luteum Bos taurus
1.14.15.6 225000
-
 sedimentation equilibrium Bos taurus
1.14.15.6 400000
-
 native-PAGE Bos taurus
1.14.15.6 415000
-
 sedimentation equilibrium Bos taurus
1.14.15.6 850000
-
 adrenal mitochondria Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.6 cholesterol + 6 reduced adrenodoxin + 3 O2 Bos taurus first step in biosynthesis of all steroid hormones pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6 several ion exchange and heptyl-Sepharose hydrophobic chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal gland
-
 Bos taurus
-
1.14.15.6 corpus luteum
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
1.14.15.6 0°C on ice, purified, 20 mM potassium phosphate buffer, pH 7.4, 20% glycerol, v/v, 0.1 mM EDTA, 0.1 mM dithioerythritol, 0.01% Emulgen 911, 3 weeks, without substantial loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 cholesterol + 6 reduced adrenodoxin + 3 O2 first step in biosynthesis of all steroid hormones Bos taurus pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
 ?
1.14.15.6 cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.6 ? 2,4,6,8 * 48000, SDS-PAGE, corpus luteum. x * 46000, x * 60000, x * 53000, x * 52000-53000 adrenal mitochondria Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.6 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.6 7.4
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin
-
 Bos taurus
1.14.15.6 heme 0.78 heme group/unit of 48000 Da P-450, 1 mol of iron/44000 g Bos taurus
1.14.15.6 NADPH NADPH-linked adrenodoxin reductase Bos taurus