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Literature summary extracted from

  • Nakajin, S.; Ishii, Y.; Shinoda, M.
    Binding of Triton X-100 to purified cytochrome P-450scc and enhancement of the cholesterol side chain cleavage activity (1979), Biochem. Biophys. Res. Commun., 87, 524-531.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.6 Brij 56, 76 and 96 Bos taurus
1.14.15.6 Emulgen 911 and 913 Bos taurus
1.14.15.6 Triton X-100 effect onto P-450 itself suggested Bos taurus
1.14.15.6 Tween 20
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 mitochondrial inner membrane
-
 Bos taurus 5743
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6 cholate extraction, pyrophosphate treatment, affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal cortex
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
1.14.15.6 -20°C, 50% glycerol, for months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 cholesterol + reduced adrenodoxin + O2 20- or 22-hydroxycholesterol and 20,22-dehydroxycholesterol are putative intermediates Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
1.14.15.6 additional information one-enzyme-three-step hypothesis Bos taurus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.6 7
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin
-
 Bos taurus
1.14.15.6 NADPH
-
 Bos taurus