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Literature summary extracted from

  • Hanukoglu, I.; Spitsberg, V.; Bumpus, J.A.; Dus, K.M.; Jefcoate, C.R.
    Adrenal mitochondrial cytochrome P-450scc. Cholesterol and adrenodoxin interactions at equilibrium and during turnover (1981), J. Biol. Chem., 256, 4321-4328.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.6 Phospholipid cholesterol gains access to the cytochrome within micelles Bos taurus
1.14.15.6 Tween 20 small stabilizing and binding activating effect on ternary complex of adrenodoxin-cholesterol-P-450scc Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.6 Ca2+ inhibits side chain cleavage activity optimally activated by 100 mM NaCl Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.6 0.0012
-
 reduced adrenodoxin
-
 Bos taurus
1.14.15.6 0.39
-
 cholesterol
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 mitochondrial inner membrane adrenodoxin reductase and adrenodoxin are peripheral proteins on matrix side of inner membrane, P-450scc is integral membrane protein Bos taurus 5743
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.6 Ca2+ very little Bos taurus
1.14.15.6 Mg2+
-
 Bos taurus
1.14.15.6 additional information Kd for adrenodoxin increases with increase of ions from NaCl, binding of adrenodoxin to P-450scc is more sensitive to changes in metal ion concentration than cholesterol binding, high salt concentrations cause fall-off in activity Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6 ammonium sulfate fractioning, aniline-Sepharose, adrenodoxin-Separose Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal cortex
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
1.14.15.6 -70°C, mitochondrial pellet, 30-40 mg/ml, 100 mM potassium phosphate, pH 7.3, 0.2 mM EDTA Bos taurus
1.14.15.6 4°C, purified, 50 mM potassium phosphate, pH 7.3, 0.1 mM dithiothreitol, 10% glycerol, stable a few weeks Bos taurus
1.14.15.6 purified, 25 mM potassium phosphate, pH 7.3, 0.05 mM EDTA, 0.05 mM dithiothreitol, 0.025 mM deoxycorticosterone, 0.25% Tween 20, 0.25% sodium cholate Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
1.14.15.6 cholesterol + reduced adrenodoxin + O2 + H+
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 r
1.14.15.6 additional information suggested shuttle mechanism in which adrenodoxin transfers electrons between adrenodoxin reductase and cytochrome P-450. No ternary adrenodoxin-adrenodoxin reductase-P-450 complex Bos taurus ?
-
 ?
1.14.15.6 additional information dissociation of oxidized adrenodoxin from P-450scc must be a potential rate-limiting factor Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.6 37
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.15.6 additional information
-
 increase of complex formation of adrenodoxin and cytochrome P-450scc in absence of cholesterol with decrease of temperature to 6°C, elevated temperature decreases the affinity of P-450scc for cholesterol Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin transports electrons from adrenodoxin reductase to cytochrome P-450 by shuttling Bos taurus
1.14.15.6 heme
-
 Bos taurus
1.14.15.6 NADPH NADPH-linked adrenodoxin reductase Bos taurus