BRENDA - Enzyme Database show

Cloning and expression of the rat adrenal cytochrome P-450 11B3 (CYP11B3) enzyme cDNA: preferential 18-hydroxylation over 11beta-hydroxylation of DOC

Zhou, M.Y.; Gomez-Sanchez, E.P.; Foecking, M.F.; Gomez-Sanchez, C.E.; Mol. Cell. Endocrinol. 114, 137-145 (1995)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.15.4
CYP11B3 enzyme cDNA cloned into and expressed in COS-7 cells
Rattus norvegicus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.15.4
G59S
mutation in CYP11B3, 5-6fold reduction of activity
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.15.4
mitochondrion
CYP11B1 in zona fasciculata, CYP11B2 in zona glomerulosa, new CYP11B3
Rattus norvegicus
5739
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.4
Rattus norvegicus
-
neonatal and adult
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.15.4
adrenal cortex
-
Rattus norvegicus
-
1.14.15.4
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.4
11-deoxycorticosterone + reduced adrenal ferredoxin + O2
18-/11beta-hydroxylation
285410
Rattus norvegicus
18-hydroxycorticosterone + oxidized adrenal ferredoxin + H2O
-
285410
Rattus norvegicus
?
1.14.15.4
11-deoxycorticosterone + reduced adrenal ferredoxin + O2
18-/11beta-hydroxylation
285410
Rattus norvegicus
corticosterone + oxidized adrenal ferredoxin + H2O
-
285410
Rattus norvegicus
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.4
CYP11B3 enzyme cDNA cloned into and expressed in COS-7 cells
Rattus norvegicus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.15.4
G59S
mutation in CYP11B3, 5-6fold reduction of activity
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.15.4
mitochondrion
CYP11B1 in zona fasciculata, CYP11B2 in zona glomerulosa, new CYP11B3
Rattus norvegicus
5739
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.15.4
adrenal cortex
-
Rattus norvegicus
-
1.14.15.4
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.4
11-deoxycorticosterone + reduced adrenal ferredoxin + O2
18-/11beta-hydroxylation
285410
Rattus norvegicus
18-hydroxycorticosterone + oxidized adrenal ferredoxin + H2O
-
285410
Rattus norvegicus
?
1.14.15.4
11-deoxycorticosterone + reduced adrenal ferredoxin + O2
18-/11beta-hydroxylation
285410
Rattus norvegicus
corticosterone + oxidized adrenal ferredoxin + H2O
-
285410
Rattus norvegicus
?