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Literature summary extracted from

  • Delorme, C.; Piffeteau, A.; Viger, A.; Marquet, A.
    Inhibition of bovine cytochrome P-45011beta by 18-unsaturated progesterone derivatives (1995), Eur. J. Biochem., 232, 247-256.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.4 lipid extract from adrenocortical mitochondria, increase of aldosterone production from corticosterone Bos taurus

Application

EC Number Application Comment Organism
1.14.15.4 medicine inhibitors for P-45011beta, against aldosterone overproduction which leads to oedematous diseases and hypertension Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.4 2 P-45011beta cDNAs, pc P-450 11beta-3 and -2, cloned into and expressed in COS-7 cells Bos taurus

General Stability

EC Number General Stability Organism
1.14.15.4 phospholipid vesicles, 0.2% soybean lecithin, added to purified P-45011beta for storage and during incubation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.4 18-ethynylprogesterone mechanism-based inhibition; weaker than 18-vinylprogesterone, inhibitor of aldosterone synthesis for both activities, stronger for 18-hydroxylation inhibition Bos taurus
1.14.15.4 18-vinylprogesterone competitive, potent inhibitor of aldosterone synthesis for both activities, 18-hydroxylation more effected, 65% inhibition of corticosterone and 11-deoxy-18-hydroxycorticosterone production by 0.03 mM; mechanism-based inhibition Bos taurus
1.14.15.4 corticosterone competitive inhibition of 11beta-hydroxylase activity Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.4 additional information
-
 additional information kinetic parameters only slightly modified by presence of phospholipids, 0.2% soybean lecithin Bos taurus
1.14.15.4 0.00087
-
 11-deoxycorticosterone without phospholipids Bos taurus
1.14.15.4 0.0011
-
 11-deoxycorticosterone with phospholipids Bos taurus
1.14.15.4 0.0015
-
 11-deoxycorticosterone +/-0.00008 Bos taurus
1.14.15.4 0.0084
-
 corticosterone without phospholipids Bos taurus
1.14.15.4 0.009
-
 corticosterone +/-0.0009 Bos taurus
1.14.15.4 0.087
-
 corticosterone with phospholipids Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.4 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.4 48500
-
 x * 48500 major form, x * 49500 isoform, SDS-PAGE Bos taurus
1.14.15.4 49500
-
 x * 48500 major form, x * 49500 isoform, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.4 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.4 copurification of P-450 11beta-2 and 11beta-3, ammonium sulfate precipitation, octyl-Sepharose, separation of 2 isoforms by hydroxyapatite column connected to an FPLC system Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.4 adrenal cortex
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
1.14.15.4 -80°C, after ammonium sulfate precipitation, 50 mM potassium phosphate, pH 7.4, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.5% sodium cholate, 0.5% soybean lecithin, several months, without loss of activity Bos taurus
1.14.15.4 -80°C, after hydroxyapatite column/FPLC system, 50 mM potassium phosphate, pH 7.4, 0.01% sodium cholate, 0.05% soybean lecithin, 0.01 mM 11-deoxycorticosterone, 0.1 mM dithiothreitol, 0.1 mM EDTA, without loss of activity Bos taurus
1.14.15.4 -80°C, after octyl-Sepharose, deoxycorticosterone-bound, 50 mM potassium phosphate, pH 7.4, 0.5% sodium cholate, 0.2% Tween 20, 0.01 mM 11-deoxycorticosterone, 0.1 mM dithiothreitol, 0.1 mM EDTA Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.4 11-deoxycorticosterone + reduced adrenal ferredoxin + O2 11beta-hydroxylation Bos taurus corticosterone + oxidized adrenal ferredoxin + H2O
-
 ?
1.14.15.4 18-hydroxycorticosterone + reduced adrenal ferredoxin + O2
-
 Bos taurus aldosterone + oxidized adrenal ferredoxin + H2O 18-hydroxylation ?
1.14.15.4 corticosterone + reduced adrenal ferredoxin + O2 18-hydroxylation Bos taurus 18-hydroxycorticosterone + oxidized adrenal ferredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.4 ? x * 48500 major form, x * 49500 isoform, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.4 30
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.15.4 30
-
 in presence of phospholipids, catalytic activity stable to extended incubation and increased resistance of hemoprotein to denaturation Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.4 0.0378
-
 corticosterone +/-0.2268, calculatetd from Kcat/Km Bos taurus
1.14.15.4 0.66
-
 11-deoxycorticosterone +/-2.112, calculatetd from Kcat/Km Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.4 7.4
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.4 NADPH adrenodoxin and NADPH-linked adrenodoxin reductase Bos taurus