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Literature summary extracted from

  • Seybert, D.
    Lipid regulation of bovine cytochrome P45011beta activity (1990), Arch. Biochem. Biophys., 279, 188-194.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.4 phosphatidylcholine saturated acyl chains such as dipalmitoyl/dimyristoyl phosphatidylcholine Bos taurus

General Stability

EC Number General Stability Organism
1.14.15.4 inclusion into phospholipid vesicles stabilizes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.4 diphosphatidyl glycerol cardiolipin, dipalmitoyl phasphatidylcholine vesicles, 50% inhibition with 4-5 mol%, complete inhibition at 15 mol% Bos taurus
1.14.15.4 phosphatidylcholine unsaturated increasing dioleoyl/diphytanoyl phosphatidylcholine Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.4 0.005
-
 11-deoxycorticosterone dipalmitoyl-phosphatidylcholine vesicles Bos taurus
1.14.15.4 0.01
-
 11-deoxycorticosterone dipalmitoyl/diphytanoyl-phosphatidylcholine vesicles Bos taurus
1.14.15.4 0.033
-
 11-deoxycorticosterone dipalmitoyl phosphatidylcholine/diphosphatidyl glycerol vesicles Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.4 mitochondrion from zona fasciculata Bos taurus 5739
-
1.14.15.4 mitochondrion reconstitution of cytochrome P45011beta, an intrinsic protein of inner membrane, into phospholipid vesicles via detergent dialysis procedure Bos taurus 5739
-
1.14.15.4 mitochondrion from zona reticularis Bos taurus 5739
-
1.14.15.4 mitochondrion from zona glomerulosa Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.4 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.4 without ionic detergents, ammonium sulfate fractionation, hexyl agarose Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.4 a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O cytochrome P45011beta is implicated in mineralocorticoid, synthesized and secreted from zona glomerulosa, as well as glucocorticoid, secreted primarily in zonae fasciculate and reticularis, biosynthesis with multiple catalytic activities, 11beta-/18-/19-hydroxylase and 18-/19-hydroxysteroid oxidase activities Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.4 adrenal cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.4 18-hydroxycorticosterone + reduced adrenal ferredoxin + O2
-
 Bos taurus aldosterone + oxidized adrenal ferredoxin + H2O
-
 ?
1.14.15.4 19-hydroxy-11-deoxycorticosterone + reduced adrenal ferredoxin + O2
-
 Bos taurus 19-oxo-11-deoxycorticosterone + oxidized adrenal ferredoxin + H2O
-
 ?
1.14.15.4 cortisol + reduced adrenal ferredoxin + O2
-
 Bos taurus cortisone + oxidized adrenal ferredoxin + ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.4 0.133
-
 11-deoxycorticosterone dipalmitoyl phosphatidylcholine vesicles + diphosphatidyl glycerol Bos taurus
1.14.15.4 0.583
-
 11-deoxycorticosterone dipalmitoyl phosphatidylcholine vesicles Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.4 NADPH adrenodoxin and NADPH-linked adrenodoxin reductase Bos taurus