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Literature summary extracted from

  • Lauber, M.; Muller, J.
    Purification and characterization of two distinct forms of rat adrenal cytochrome P450(11) beta: functional and structural aspects (1989), Arch. Biochem. Biophys., 274, 109-119.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.4 Anti-11beta-hydroxylase IgG cross-reaction with 51 kDa protein Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.4 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.4 49000
-
 x * 49000 and x * 51000, SDS-PAGE Rattus norvegicus
1.14.15.4 51000
-
 x * 49000 and x * 51000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.4 Rattus norvegicus
-
 male, Zur:SIV low sodium, high potassium or high sodium, low potassium treated for stimulation or supprssion, respectively
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.4 octyl-Sepharose chromatography, cholate extraction Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.4 a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O 11beta-/18-hydroxylation and aldosterone synthesis are catalyzed by 2 distinct forms of cytochrome P-45011beta Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.4 adrenal cortex capsular portion in zona glomerulosa cells, decapsular in zonae fasciculata and reticularis Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.4 additional information
-
 conversion of 11-deoxycorticosterone Rattus norvegicus
1.14.15.4 0.00000314
-
 aldosterone production, mitochondria from zona glomerulosa Rattus norvegicus
1.14.15.4 0.0000236
-
 18-hydroxycorticosterone production, mitochondria from zona fasciculata Rattus norvegicus
1.14.15.4 0.000119
-
 18-hydroxycorticosterone production, mitochondria from zona glomerulosa Rattus norvegicus
1.14.15.4 0.00206
-
 18-hydroxydeoxycorticosterone production, mitochondria from zona glomerulosa Rattus norvegicus
1.14.15.4 0.00599
-
 18-hydroxydeoxycorticosterone production, mitochondria from zona fasciculata Rattus norvegicus
1.14.15.4 0.00691
-
 corticosterone production, mitochondria from zona glomerulosa Rattus norvegicus
1.14.15.4 0.00951
-
 corticosterone production, mitochondria from zona fasciculata Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.4 11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 49 kDa protein, exclusively in zona glomerulosa, 11beta -hydroxylation, as well as 18-hydroxylation and 18-hydroxydehydrogenation of corticosterone with 18-hydroxydeoxycorticosterone and 18-hydroxycorticosterone as intermediates Rattus norvegicus aldosterone + oxidized adrenal ferredoxin + 2 H2O product of 49 kDa protein ?
1.14.15.4 11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 51 kDa protein, zona fasciculata and glomerulosa, high sodium and low potassium, 11beta-/18-hydroxylation Rattus norvegicus aldosterone + oxidized adrenal ferredoxin + 2 H2O product of 49 kDa protein ?

Subunits

EC Number Subunits Comment Organism
1.14.15.4 ? x * 49000 and x * 51000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.4 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.4 7.4
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.4 NADPH
-
 Rattus norvegicus