Literature summary extracted from
Ogishima, T.; Mitani, F.; Ishimura, Y
Isolation of two distinct cytochromes P-45011beta with aldosterone synthase activity from bovine adrenocortical mitochondria (1989), J. Biochem., 105, 497-499.
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.14.15.4 |
mitochondrion |
inner membrane, all 3 zones |
Bos taurus |
5739 |
- |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.15.4 |
Bos taurus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.15.4 |
Na-cholate extraction, ammonium sulfate precipitation, aniline-Sepharose chromatography, hydroxylapatite HPLC |
Bos taurus |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.14.15.4 |
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O |
11beta-/18-hydroxylation and aldosterone synthesis are catalyzed by 2 distinct forms of cytochrome P-45011beta |
Bos taurus |
|
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.14.15.4 |
adrenal cortex |
- |
Bos taurus |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.15.4 |
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 |
11beta-hydroxylase and 18-hydroxylase activities, after replacement of Tween 20 by phosphatidylcholine catalytic activity for aldosterone activity is exhibited, form 1 higher active in aldosterone and 18-hydroxycorticosterone production and less active in the production of corticosterone and 18-hydroxydeoxycorticosterone from deoxycorticosterone as form 2 |
Bos taurus |
aldosterone + oxidized adrenal ferredoxin + 2 H2O |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.15.4 |
? |
x * 48500-51500, SDS-PAGE after Sepharose, x * 48400/49500, SDS-PAGE after HPLC |
Bos taurus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.15.4 |
cytochrome P-45011-beta |
- |
Bos taurus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.15.4 |
37 |
- |
assay at |
Bos taurus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.15.4 |
7.4 |
- |
assay at |
Bos taurus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.15.4 |
NADPH |
adrenodoxin and NADPH-linked adrenodoxin reductase |
Bos taurus |
|