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Literature summary extracted from

  • Watanuki, M.; Tilley, B.E.; Hall, P.F.
    Cytochrome P-450 for 11beta- and 18-hydroxylase activities of bovine adrenocortical mitochondria: one enzyme or two? (1978), Biochemistry, 17, 127-130.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.15.4 deoxycorticosterone stabilizes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.4 acetone 10%, v/v, complete inhibition of hydroxylation Bos taurus
1.14.15.4 antiserum produced by rabbits Bos taurus
1.14.15.4 CO
-
 Bos taurus
1.14.15.4 deoxycorticosterone competitive substrate inhibition Bos taurus
1.14.15.4 ethanol 10%, v/v, complete inhibition of hydroxylation Bos taurus
1.14.15.4 KCl total inactivation Bos taurus
1.14.15.4 methanol 10%, v/v, complete inhibition of hydroxylation Bos taurus
1.14.15.4 Metyrapone competitive substrate inhibition Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.4 mitochondrion
-
 Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.4 additional information Bos taurus corticosterone normal substrate in vivo ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.4 Bos taurus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.4 a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O both hydroxylation activities seem to occur in a single enzyme Bos taurus
a
1.14.15.4 a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O heme protein of so-called P-450 family Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.4 adrenal cortex
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.4 0.000171
-
 18-hydroxylation activity, 4% O2, 96% N2 Bos taurus
1.14.15.4 0.000211
-
 18-hydroxylation activity, 21% O2, 79% N2 Bos taurus
1.14.15.4 0.00205
-
 11beta-hydroxylation activity, 4% O2, 96% N2 Bos taurus
1.14.15.4 0.00278
-
 11beta-hydroxylation activity, 21% O2, 79% N2 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.14.15.4 4°C, 11 days, both activities decrease considerably in a similar way, deoxycorticosterone delays decline Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.4 11-deoxycorticosterone + reduced adrenal ferredoxin + O2 addition of adrenodoxin and adrenodoxin reductase Bos taurus corticosterone + oxidized adrenal ferredoxin + H2O
-
 ?
1.14.15.4 corticosterone + reduced adrenal ferredoxin + O2 addition of adrenodoxin and adrenodoxin reductase Bos taurus 18-hydroxy-11-deoxycorticosterone + oxidized adrenal ferredoxin + ?
-
 ?
1.14.15.4 additional information corticosterone normal substrate in vivo Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.4 20
-
-
 Bos taurus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.15.4 2 50 at 50°C almost no activity Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.15.4 2
-
 14% residual activity of 11beta- and 18-hydroxylation Bos taurus
1.14.15.4 30
-
 after preincubation for 20 min, 27% residual activity, after 60 min 5.7% Bos taurus
1.14.15.4 50
-
 complete inactivation Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.4 NADPH
-
 Bos taurus