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Literature summary extracted from

  • Honeck, H.; Schunck, W.H.; Riege, P.; Muller, H.G.
    The cytochrome P-450 alkane monooxygenase system of the yeast Lodderomyces elongisporus: purification and some properties of the NADPH-cytochrome P-450 reductase (1982), Biochem. Biophys. Res. Commun., 106, 1318-1324.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.2.4 Nonionic detergent activation Lodderomyces elongisporus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 microsome
-
 Lodderomyces elongisporus
-
-
1.14.15.3 cell membrane membrane-bound enzyme Lodderomyces elongisporus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 79000
-
 x * 79000, SDS-PAGE Lodderomyces elongisporus
1.14.15.3 79000
-
 SDS-PAGE Lodderomyces elongisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 additional information Lodderomyces elongisporus acts as an electron transfer component of the alkane monoxygenase system ?
-
 ?
1.6.2.4 additional information Lodderomyces elongisporus EH15D acts as an electron transfer component of the alkane monoxygenase system ?
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Lodderomyces elongisporus
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Lodderomyces elongisporus EH15D
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Lodderomyces elongisporus
-
-
-
1.14.15.3 Lodderomyces elongisporus
-
-
-
1.14.15.3 Lodderomyces elongisporus EH15D
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 DEAE-Sephacel and hydroxylapatite column chromatography Lodderomyces elongisporus
1.14.15.3
-
 Lodderomyces elongisporus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 18.4
-
 electron acceptor: 2,6-dichlorophenolindophenol Lodderomyces elongisporus
1.6.2.4 68.9
-
-
 Lodderomyces elongisporus
1.6.2.4 98.3
-
 electron acceptor: ferricyanide Lodderomyces elongisporus

Storage Stability

EC Number Storage Stability Organism
1.6.2.4 -20°C Lodderomyces elongisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Lodderomyces elongisporus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Lodderomyces elongisporus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Lodderomyces elongisporus EH15D 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Lodderomyces elongisporus EH15D 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 additional information acts as an electron transfer component of the alkane monoxygenase system Lodderomyces elongisporus ?
-
 ?
1.6.2.4 additional information acts as an electron transfer component of the alkane monoxygenase system Lodderomyces elongisporus EH15D ?
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Lodderomyces elongisporus n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Lodderomyces elongisporus EH15D n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-hexadecane + NADPH + H+ + O2 NADPH-cyt P-450-reductase Lodderomyces elongisporus n-hexadecanol + NADP+ + H2O
-
 ?
1.14.15.3 n-hexadecane + NADPH + H+ + O2 NADPH-cyt P-450-reductase Lodderomyces elongisporus EH15D n-hexadecanol + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.4 ? x * 79000, SDS-PAGE Lodderomyces elongisporus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD ratio FAD : FMN 1 : 1 Lodderomyces elongisporus
1.6.2.4 FMN 1 mol per mol of enzyme Lodderomyces elongisporus
1.6.2.4 FMN ratio FAD : FMN 1 : 1 Lodderomyces elongisporus
1.6.2.4 NADPH
-
 Lodderomyces elongisporus
1.14.15.3 NADPH
-
 Lodderomyces elongisporus