Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Griffith, G.R.; Ruettinger, R.T.; McKenna, E.J.; Coon, M.J.
    Fatty acid omega-hydroxylase (alkane hydroxylase) from Pseudomonas oleovorans (1978), Methods Enzymol., 53, 356-360.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.3 KCN reversible inhibitor Pseudomonas oleovorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.3 cell membrane membrane-bound enzyme, needs 20 molecules phospholipid per polypeptide chain Pseudomonas oleovorans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.3 Fe 1 atom Fe per 42000 g/mol peptide Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Pseudomonas oleovorans
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Pseudomonas oleovorans
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.14.15.3 ferrous ions restore activity after treatment with EDTA and dithionite Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.3 2
-
-
 Pseudomonas oleovorans

Storage Stability

EC Number Storage Stability Organism
1.14.15.3 -20°C, 1 month, under 50% activity retained Pseudomonas oleovorans
1.14.15.3 -70°C, 12 days, over 90% activity retained Pseudomonas oleovorans
1.14.15.3 -70°C, 6 months, over 50% activity retained Pseudomonas oleovorans
1.14.15.3 4°C, 5 days, under 50% activity retained Pseudomonas oleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 1,7-octadiene + NADH + H+ + O2 epoxidation of simple, aliphatic terminal olefins Pseudomonas oleovorans 1,2-epoxy-7-octene + NAD+ + H2O
-
 ?
1.14.15.3 2,5-dimethylhexane + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
1.14.15.3 cyclohexane + NADH + H+ + O2
-
 Pseudomonas oleovorans cyclohexanol + NAD+ + H2O
-
 ?
1.14.15.3 fatty acid + NAD(P)H + H+ + O2 chain length C6-C14 Pseudomonas oleovorans omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
1.14.15.3 methylcyclohexane + NADH + H+ + O2
-
 Pseudomonas oleovorans ?
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 rubredoxin is electron carrier Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 chain length C6-C14 Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.3 30
-
 assay conditions Pseudomonas oleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.3 7.4
-
 assay conditions Pseudomonas oleovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.3 NADH hydroxylation needs also a NADH-dependent bacterial reductase Pseudomonas oleovorans