Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ruettiger, R.T.; Olson, S.T.; Boyer, R.F.; Coon, M.J.
    Identification of the omega-hydroxylase of Pseudomonas oleovorans as a nonheme iron protein requiring phospholipid for catalytic activity (1974), Biochem. Biophys. Res. Commun., 57, 1011-1017.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.3 Phospholipid enhances enzyme activity Pseudomonas oleovorans

General Stability

EC Number General Stability Organism
1.14.15.3 treatment with 0.1 M EDTA and 0.1 M dithionite reduces activity to 10% Pseudomonas oleovorans
1.14.15.3 treatment with sodium cholate and subsequent precipitation with ammonium sulfate reduces activity to 40% Pseudomonas oleovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.3 Fe non-heme iron protein Pseudomonas oleovorans
1.14.15.3 Fe 1 atom Fe per 42000 g/mol peptide Pseudomonas oleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.3 42000
-
 x * 42000, SDS-PAGE Pseudomonas oleovorans
1.14.15.3 2000000
-
-
 Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Pseudomonas oleovorans
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Pseudomonas oleovorans
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.14.15.3 addition of dilauroylglyceryl-3-phosphorylcholine restores initial activity after treatment with sodium cholate and subsequent precipitation with ammonium sulfate Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.3 0.59
-
-
 Pseudomonas oleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 fatty acid + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 rubredoxin is electron carrier Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.3 ? x * 42000, SDS-PAGE Pseudomonas oleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.3 30
-
 assay conditions Pseudomonas oleovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.3 NADH hydroxylation needs also a NADH-dependent bacterial reductase Pseudomonas oleovorans