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Literature summary extracted from

  • Cardini, G.; Jurtshuk, P.
    The enzymatic hydroxylation of n-octane by Corybacterium sp. strain 7E1C (1970), J. Biol. Chem., 245, 2789-2796.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.3 8-hydroxyquinoline 1 mM, 2 min 100% inhibition Corynebacterium sp.
1.14.15.3 CO 50 : 50 100% inhibition, 10 : 90 91% inhibition Corynebacterium sp.
1.14.15.3 Cu2+ 0.1 mM concentration, 100% inhibition Corynebacterium sp.
1.14.15.3 Fe2+ in 0.5 mM concentration, 100% inhibition Corynebacterium sp.
1.14.15.3 Hg2+ 0.1 mM concentration, 100% inhibition Corynebacterium sp.
1.14.15.3 KCN 1 mM, 2 min 32% inhibition Corynebacterium sp.
1.14.15.3 additional information no effect: FAD and FMN Corynebacterium sp.
1.14.15.3 p-chloromercuribenzoate 0.1 mM concentration, 51% inhibition Corynebacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.3 Fe heme protein Corynebacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Corynebacterium sp.
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Corynebacterium sp. 7E1C
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Corynebacterium sp.
-
-
-
1.14.15.3 Corynebacterium sp. 7E1C
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.3
-
 Corynebacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.3 cell suspension culture
-
 Corynebacterium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.3 4.26
-
-
 Corynebacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Corynebacterium sp. n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Corynebacterium sp. 7E1C n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-octane + NADH + H+ + O2 cytochrome P450 is electron carrier Corynebacterium sp. n-octanol + NAD+ + H2O
-
 ?
1.14.15.3 n-octane + NADH + H+ + O2 cytochrome P450 is electron carrier Corynebacterium sp. 7E1C n-octanol + NAD+ + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.3 30
-
 assay conditions Corynebacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.3 7.4
-
 assay conditions Corynebacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.3 NAD+ very little activity at 1 mM level Corynebacterium sp.
1.14.15.3 NADH maximal activity at 1 mM concentration Corynebacterium sp.
1.14.15.3 NADPH very little activity at 1 mM level Corynebacterium sp.