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Literature summary extracted from

  • Small, F.J.; Ensign, S.A.
    Alkene monooxygenase from Xanthobacter strain Py2. Purification and characterization of a four-component system central to the bacterial metabolism of aliphatic alkenes (1997), J. Biol. Chem., 272, 24913-24920.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.69 propyne mechanism-based inactivator of the 21200 Da protein Xanthobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.69 Iron enzyme contains 4 components: 1. a monomeric 35500 Da NADH reductase containing 1 mol of FAD and a probable 2Fe-2S center, 2. a 13300 Da ferredoxin containing a Rieske-type 2Fe-2S cluster, 3. a 11000 Da monomeric protein that contains no detectable cofactors, 4. a 212000 Da alpha2beta2gamma2 multimeric protein containing 4 atoms of nonheme iron. The physiological electron acceptor for the reductase is the Rieske-type ferredoxin, which is proposed to be an intermediate electron carrier between the reductase and terminal catalytic component of the system Xanthobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.69 propene + NADH + H+ + O2 Xanthobacter sp. the inducible enzyme is central to the bacterial metabolism of aliphatic alkenes. Enzyme is expressed during growth of Xanthobacter on aliphatic alkenes or epoxides and repressed during growth on other carbon sources 1,2-epoxypropane + NAD+ + H2O
-
 ?
1.14.13.69 propene + NADH + H+ + O2 Xanthobacter sp. Py2 the inducible enzyme is central to the bacterial metabolism of aliphatic alkenes. Enzyme is expressed during growth of Xanthobacter on aliphatic alkenes or epoxides and repressed during growth on other carbon sources 1,2-epoxypropane + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.69 Xanthobacter sp.
-
-
-
1.14.13.69 Xanthobacter sp. Py2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.69
-
 Xanthobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.69 additional information
-
-
 Xanthobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.69 propene + NADH + H+ + O2
-
 Xanthobacter sp. 1,2-epoxypropane + NAD+ + H2O
-
 ?
1.14.13.69 propene + NADH + H+ + O2 the inducible enzyme is central to the bacterial metabolism of aliphatic alkenes. Enzyme is expressed during growth of Xanthobacter on aliphatic alkenes or epoxides and repressed during growth on other carbon sources Xanthobacter sp. 1,2-epoxypropane + NAD+ + H2O
-
 ?
1.14.13.69 propene + NADH + H+ + O2
-
 Xanthobacter sp. Py2 1,2-epoxypropane + NAD+ + H2O
-
 ?
1.14.13.69 propene + NADH + H+ + O2 the inducible enzyme is central to the bacterial metabolism of aliphatic alkenes. Enzyme is expressed during growth of Xanthobacter on aliphatic alkenes or epoxides and repressed during growth on other carbon sources Xanthobacter sp. Py2 1,2-epoxypropane + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.69 More enzyme contains 4 components: 1. a monomeric 35500 Da NADH reductase containing 1 mol of FAD and a probable 2Fe-2S center, mass spectrometry 2. a dimeric ferredoxin consisting of two 13300 Da subunits, each containing a Rieske-type 2Fe-2S cluster, SDS-PAGE 3. a 11000 Da monomeric protein that contains no detectable cofactors, mass spectrometry 4. a 212000 Da alpha2beta2gamma2 multimeric protein containing 4 atoms of nonheme iron Xanthobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.69 FAD the 35500 Da NADH reductase component contains 1 mol of FAD Xanthobacter sp.