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Literature summary extracted from

  • Ogishima, T.; Deguchi, S.; Okuda, K.
    Purification and characterization of cholesterol 7alpha-hydroxylase from rat liver microsomes (1987), J. Biol. Chem., 262, 7646-7650.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.23 additional information
-
additional information
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.23 microsome
-
Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.23 52000
-
x * 52000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.23 cholesterol + [reduced NADPH-hemoprotein reductase] + O2 Rattus norvegicus rate-limiting step in bile acid formation 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.23 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.23
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.23 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.23 156
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.23 5alpha-cholestan-3beta-ol + NADPH + O2
-
Rattus norvegicus 5alpha-cholestan-3beta,7alpha-diol + NADP+ + H2O
-
?
1.14.14.23 cholesterol + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation of cholesterol with strict regio- and stereoselectivity, inert towards other sterols and intermediates in conversion of cholesterol to bile acids Rattus norvegicus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.23 cholesterol + [reduced NADPH-hemoprotein reductase] + O2 rate-limiting step in bile acid formation Rattus norvegicus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.14.23 ? x * 52000, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.23 0.833
-
cholesterol
-
Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.23 7.4
-
-
Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.23 heme 9 nmol of cytochrome P-450 per mg of protein Rattus norvegicus
1.14.14.23 NADPH
-
Rattus norvegicus