Any feedback?
Literature summary extracted from
- Regulation of cholesterol 7alpha-hydroxylase in the liver. Purification of cholesterol 7alpha-hydroxylase and the immunochemical evidence for the induction of cholesterol 7alpha-hydroxylase by cholestyramine and circadian rhythm (1990), J. Biol. Chem., 265, 3889-3897.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.23 | Triton X-100 | stimulates | Rattus norvegicus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.23 | 0.031 | - |
cholesterol | isoenzyme II | Rattus norvegicus | |
| 1.14.14.23 | 0.052 | - |
cholesterol | isoenzyme I | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.23 | microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.23 | 53000 | - |
x * 53000, SDS-PAGE | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.23 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.23 | 2 isoenzymes: I and II | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.23 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.23 | 36.7 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.23 | cholesterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rattus norvegicus | 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.23 | ? | x * 53000, SDS-PAGE | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.23 | 0.0417 | - |
cholesterol | isoenzyme I | Rattus norvegicus | |
| 1.14.14.23 | 0.0778 | - |
cholesterol | isoenzyme II | Rattus norvegicus | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.23 | heme | cytochrome P-450 dependent enzyme | Rattus norvegicus | |
| 1.14.14.23 | heme | 8 nmol of heme per mg of protein | Rattus norvegicus | |
| 1.14.14.23 | NADPH | - |
Rattus norvegicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
