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Literature summary extracted from

  • Chebrou, H.; Hurtubise, Y.; Barriault, D.; Sylvestre, M.
    Heterologous expression and characterization of the purified oxygenase component of Rhodococcus globerulus P6 biphenyl dioxygenase and of chimeras derived from it (1999), J. Bacteriol., 181, 4805-4811.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.18 in Escherichia coli strain M15 and SG13009 Rhodococcus globerulus
1.14.12.18 in Pseudomonas putida strain KT2442 Rhodococcus globerulus

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.18 Rhodococcus globerulus
-
-
-
1.14.12.18 Rhodococcus globerulus P6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.18 His-tagged ISP, expressed in Escherichia coli and Pseudomonas putida Rhodococcus globerulus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.18 2,2'-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
 ?
1.14.12.18 2,2'-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus P6 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
 ?
1.14.12.18 2,5-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus cis-2',3'-dihydroxy-1'-(2,5-dichlorophenyl)-cyclohexa-4',6'-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
 ?
1.14.12.18 2,5-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus P6 cis-2',3'-dihydroxy-1'-(2,5-dichlorophenyl)-cyclohexa-4',6'-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
 ?
1.14.12.18 3,3'-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 4,5-dihydroxy-1-phenylcyclohexa-1,2-diene + NAD+ + HCl
-
 ?
1.14.12.18 3,3'-dichlorobiphenyl + NADH + O2
-
 Rhodococcus globerulus P6 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 4,5-dihydroxy-1-phenylcyclohexa-1,2-diene + NAD+ + HCl
-
 ?
1.14.12.18 biphenyl + NAD(P)H + O2
-
 Rhodococcus globerulus 2,3-dihydro-dihydroxybiphenyl + NAD(P)+
-
 ?
1.14.12.18 biphenyl + NAD(P)H + O2
-
 Rhodococcus globerulus P6 2,3-dihydro-dihydroxybiphenyl + NAD(P)+
-
 ?