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Literature summary extracted from

  • Gardner, A.M.; Martin, L.A.; Gardner, P.R.; Dou, Y.; Olson, J.S.
    Steady-state and transient kinetics of Escherichia coli nitric-oxide dioxygenase (flavohemoglobin). The B10 tyrosine hydroxyl is essential for dioxygen binding and catalysis (2000), J. Biol. Chem., 275, 12581-12589.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.17 0.00011
-
 NO at 20°C Escherichia coli
1.14.12.17 0.00028
-
 NO at 37°C Escherichia coli
1.14.12.17 0.0032
-
 NADH at 20°C Escherichia coli
1.14.12.17 0.0048
-
 NADH at 37°C Escherichia coli
1.14.12.17 0.013
-
 O2 with 0.0001 mM NO at 20°C Escherichia coli
1.14.12.17 0.027
-
 O2 with 0.001 mM NO at 20°C Escherichia coli
1.14.12.17 0.035
-
 O2 with 0.0001 mM NO at 37°C Escherichia coli
1.14.12.17 0.1
-
 O2 with 0.001 mM NO at 37°C Escherichia coli
1.14.12.17 0.18
-
 NADPH at 20°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.17 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.17 NO + O2 + NAD(P)H
-
 Escherichia coli NO3- + NAD(P)+ + H+
-
 ?
1.14.12.17 NO + O2 + NADH
-
 Escherichia coli NO3- + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.17 flavoHb
-
 Escherichia coli
1.14.12.17 flavohemoglobin
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.12.17 50
-
 NADH per heme at 37°C Escherichia coli
1.14.12.17 83
-
 NADH per heme at 20°C Escherichia coli
1.14.12.17 94
-
 NO per heme at 20°C Escherichia coli
1.14.12.17 670
-
 NO per heme at 37°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.17 FAD apparent Kd of 40 nM Escherichia coli