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Literature summary extracted from

  • Choroba, O.W.; Williams, D.H.; Spencer, J.B.
    Biosynthesis of the vancomycin group of antibiotics: involvement of an unusual dioxygenase in the pathway to (S)-4-hydroxyphenylglycine (2000), J. Am. Chem. Soc., 122, 5389-5390.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.46 expressed in Escherichia coli Amycolatopsis orientalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.46 Fe2+ putative FeIV=O species hydroxylates benzylic position Amycolatopsis orientalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.46 40368
-
 alpha,alpha, 2 * 40368, gel filtration, electrospray mass spectrometry Amycolatopsis orientalis
1.13.11.46 80000
-
 gel filtration, electrospray mass spectrometry Amycolatopsis orientalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.46 4-hydroxyphenylpyruvate + O2 Amycolatopsis orientalis
-
 4-hydroxymandelate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.46 Amycolatopsis orientalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.46 recombinant His-tagged protein, Ni-NTA resin Amycolatopsis orientalis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.13.11.46 4-hydroxyphenylpyruvate + O2 = (S)-4-hydroxymandelate + CO2 one oxygen from molecular oxygen is incorporated into the carboxyl group and one into the benzylic hydroxyl group Amycolatopsis orientalis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.46 4-hydroxyphenylpyruvate + O2
-
 Amycolatopsis orientalis 4-hydroxymandelate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.46 dimer alpha,alpha, 2 * 40368, gel filtration, electrospray mass spectrometry Amycolatopsis orientalis