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Literature summary extracted from

  • Su, C.; Brodowsky, I.D.; Oliw, E.H.
    Studies on linoleic acid 8R-dioxygenase and hydroperoxide isomerase of the fungus Gaeumannomyces graminis (1995), Lipids, 30, 43-50.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.11.60 8R-dioxygenase is unstable during purification. It can be stabilized by glutathione, glutathione peroxidase and ethylenediaminetetraacetic acid Gaeumannomyces graminis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.60 4-aminobenzamidine 1 mM, over 70% inhibition Gaeumannomyces graminis
1.13.11.60 acetonitrile 10%, inactivation Gaeumannomyces graminis
1.13.11.60 Emulgen 911 0.5%, inactivation Gaeumannomyces graminis
1.13.11.60 glycine 2% Gaeumannomyces graminis
1.13.11.60 ICI 230,487 0.03 mM, 58% inhibition Gaeumannomyces graminis
1.13.11.60 Isopropanol 10%, inactivation Gaeumannomyces graminis
1.13.11.60 linoleic acid 0.1 mM, partial inactivation, may indicate product inhibition Gaeumannomyces graminis
1.13.11.60 additional information sorbitol (0.75 M, 14%), glycerol (15%) and ethylene glycol (25%) do not inhibit the enzyme Gaeumannomyces graminis
1.13.11.60 N-tosyl-L-phenylalanyl chloromethyl ketone 1 mM, over 70% inhibition Gaeumannomyces graminis
1.13.11.60 paracetamol 0.03 mM, 44% inhibition Gaeumannomyces graminis
1.13.11.60 polyoxyethylene-10 lauryl ether 0.5%, inactivation Gaeumannomyces graminis
1.13.11.60 sodium deoxycholate 0.5%, inactivation Gaeumannomyces graminis
1.13.11.60 Triton X-100 0.5%, inactivation Gaeumannomyces graminis
1.13.11.60 Tween 20 0.5%, inactivation Gaeumannomyces graminis
1.13.11.60 Tween 80 0.5%, inactivation Gaeumannomyces graminis
1.13.11.60 Urea 1-4 M Gaeumannomyces graminis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.92 microsome
-
 Gaeumannomyces graminis
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.60 linoleate + O2 Gaeumannomyces graminis
-
 (8R,10E,12Z)-8-hydroperoxy-10,12-octadecadienoate
-
 ?
5.4.4.6 (8R,9Z,12Z)-8-hydroperoxy-10,12-octadecadienoate Gaeumannomyces graminis
-
 (7S,8S,9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.60 Gaeumannomyces graminis
-
-
-
4.2.1.92 Gaeumannomyces graminis
-
-
-
5.4.4.6 Gaeumannomyces graminis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.60 partial Gaeumannomyces graminis
5.4.4.6 partial Gaeumannomyces graminis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.92 mycelium
-
 Gaeumannomyces graminis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.60 linoleate + O2
-
 Gaeumannomyces graminis (8R,10E,12Z)-8-hydroperoxy-10,12-octadecadienoate
-
 ?
4.2.1.92 (8R)-hydroperoxy-(9Z,12Z)-octadecadienoic acid
-
 Gaeumannomyces graminis (7S,8S)-dihydroxy-(9Z,12Z)-octadecadienoic acid + H2O
-
 ?
5.4.4.6 (8R,9Z,12Z)-8-hydroperoxy-10,12-octadecadienoate
-
 Gaeumannomyces graminis (7S,8S,9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.60 linoleic acid 8R-dioxygenase
-
 Gaeumannomyces graminis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.60 7.4
-
 assay at Gaeumannomyces graminis
4.2.1.92 7 10
-
 Gaeumannomyces graminis
5.4.4.6 7 10 broad optimum Gaeumannomyces graminis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.60 Gaeumannomyces graminis isoelectric focusing 6.3 6.1
5.4.4.6 Gaeumannomyces graminis isoelectric focusing 6.3 6.1