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Literature summary extracted from

  • Brodowsky, I.D.; Hamberg, M.; Oliw, E.H.
    BW A4C and other hydroxamic acids are potent inhibitors of linoleic acid 8R-dioxygenase of the fungus Gaeumannomyces graminis (1994), Eur. J. Pharmacol., 254, 43-47.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.60 1,10-phenanthroline 2.5 mM, 50% inhibition Gaeumannomyces graminis
1.13.11.60 2-mercaptoethanol
-
 Gaeumannomyces graminis
1.13.11.60 diclofenac 0.2 mM, over 80% inhibition Gaeumannomyces graminis
1.13.11.60 dithiothreitol
-
 Gaeumannomyces graminis
1.13.11.60 eicosatetraynoic acid
-
 Gaeumannomyces graminis
1.13.11.60 linoleate-hydroxamic acid
-
 Gaeumannomyces graminis
1.13.11.60 additional information GSH (1 mM), GSSG (1 mM), EDTA (1 or 10 mM), EGTA (1 or 10 mM) and H202 (10 mM) are without effect on the 8R-dioxygenase Gaeumannomyces graminis
1.13.11.60 N-[(E)-3-(3-phenoxyphenyl)prop-2-enyl]acetohydroxamic acid i.e. BW A4C Gaeumannomyces graminis
1.13.11.60 nordihydroguaiaretic acid
-
 Gaeumannomyces graminis
1.13.11.60 paracetamol 0.2 mM, over 80% inhibition Gaeumannomyces graminis
1.13.11.60 phenylmethanesulfonyl fluoride 0.2 mM, inhibits the 8R-dioxygenase enzyme by over 85% Gaeumannomyces graminis
1.13.11.60 zileuton
-
 Gaeumannomyces graminis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.60 linoleate + O2 Gaeumannomyces graminis
-
 (8R,10E,12Z)-8-hydroperoxy-10,12-octadecadienoate
-
 ?
5.4.4.6 (8R,9Z,12Z)-8-hydroperoxy-10,12-octadecadienoate Gaeumannomyces graminis
-
 (7S,8S,9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.60 Gaeumannomyces graminis
-
-
-
4.2.1.92 Gaeumannomyces graminis
-
-
-
5.4.4.6 Gaeumannomyces graminis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.60 mycelium
-
 Gaeumannomyces graminis
-
5.4.4.6 mycelium
-
 Gaeumannomyces graminis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.60 linoleate + O2
-
 Gaeumannomyces graminis (8R,10E,12Z)-8-hydroperoxy-10,12-octadecadienoate
-
 ?
4.2.1.92 (8R)-hydroperoxy-(9Z,12Z)-octadecadienoic acid
-
 Gaeumannomyces graminis (7S,8S)-dihydroxy-(9Z,12Z)-octadecadienoic acid + H2O
-
 ?
5.4.4.6 (8R,9Z,12Z)-8-hydroperoxy-10,12-octadecadienoate
-
 Gaeumannomyces graminis (7S,8S,9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.60 7.2 7.6
-
 Gaeumannomyces graminis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.60 5 9 stable Gaeumannomyces graminis
5.4.4.6 5 9 stable Gaeumannomyces graminis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.11.60 0.002
-
 pH and temperature not specified in the publication Gaeumannomyces graminis N-[(E)-3-(3-phenoxyphenyl)prop-2-enyl]acetohydroxamic acid
1.13.11.60 0.003 0.01 pH and temperature not specified in the publication Gaeumannomyces graminis zileuton
1.13.11.60 0.02
-
 pH and temperature not specified in the publication Gaeumannomyces graminis linoleate-hydroxamic acid
1.13.11.60 0.09
-
 pH and temperature not specified in the publication Gaeumannomyces graminis nordihydroguaiaretic acid
1.13.11.60 0.1
-
 pH and temperature not specified in the publication Gaeumannomyces graminis dithiothreitol
1.13.11.60 0.15
-
 pH and temperature not specified in the publication Gaeumannomyces graminis eicosatetraynoic acid