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Literature summary extracted from

  • Su, C.; Oliw, E.H.
    Purification and characterization of linoleate 8-dioxygenase from the fungus Gaeumannomyces graminis as anovel hemoprotein (1996), J. Biol. Chem., 271, 14112-14118.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.11.60 repeated freezing and thawing, particularly of dilute enzyme solutions, inactivates the enzyme Gaeumannomyces graminis
1.13.11.60 the enzyme is quite unstable at the early steps of purification, but active fractions from anion-exchange chromatography can be kept for 1 week on ice with only a small loss of enzyme activity Gaeumannomyces graminis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.60 additional information not inhibited by 1 mM KCN. CO does not inhibit the enzyme Gaeumannomyces graminis
1.13.11.60 N-[(E)-3-(3-phenoxyphenyl)prop-2-enyl]acetohydroxamic acid i.e. BW A4C, more than 90% inhibition at 0.01 mM, about 50% inhibition at 0.001 mM Gaeumannomyces graminis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.60 0.008
-
 linoleate pH 7.4 25°C Gaeumannomyces graminis
1.13.11.60 0.03
-
 O2 pH 7.4 25°C Gaeumannomyces graminis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.60 130000
-
 4 * 130000, gel filtration Gaeumannomyces graminis
1.13.11.60 520000
-
 gel filtration Gaeumannomyces graminis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.60 linoleate + O2 Gaeumannomyces graminis
-
 (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.13.11.60 urea the enzyme can be incubated in 4 M urea for 1 h, followed by desalting, with only partial loss of enzyme activity Gaeumannomyces graminis

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.60 Gaeumannomyces graminis Q9UUS2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.60
-
 Gaeumannomyces graminis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.60 mycelium
-
 Gaeumannomyces graminis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.60 1.8
-
 pH 7.4, 0°C Gaeumannomyces graminis

Storage Stability

EC Number Storage Stability Organism
1.13.11.60 -80°C, stable Gaeumannomyces graminis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.60 linoleate + O2
-
 Gaeumannomyces graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?
1.13.11.60 linoleate + O2 H2O2 (up to 9 mM) does not support enzyme activity under anaerobic conditions Gaeumannomyces graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.60 tetramer 4 * 130000, gel filtration Gaeumannomyces graminis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.60 15
-
 linoleate pH 7.4, 0°C Gaeumannomyces graminis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.60 7.2 7.4
-
 Gaeumannomyces graminis

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.60 heme the enzyme contains at least 2 mol of heme/mol of enzyme Gaeumannomyces graminis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.60 Gaeumannomyces graminis chromatofocusing
-
 5.2