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Literature summary extracted from

  • Kenyon, G.L.; Hegeman, G.D.
    Mandelate racemase (1979), Adv. Enzymol. Relat. Areas Mol. Biol., 50, 325-360.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.2.2 1-hydroxy-2-naphthoic acid reversible Pseudomonas putida
5.1.2.2 3-Hydroxy-2-naphthoic acid reversible Pseudomonas putida
5.1.2.2 4-Hydroxycoumarin reversible Pseudomonas putida
5.1.2.2 Anthranil-3-carboxylic acid reversible Pseudomonas putida
5.1.2.2 Coumarilic acid reversible Pseudomonas putida
5.1.2.2 DL-alpha-Phenylglycidate
-
 Pseudomonas putida
5.1.2.2 EDTA
-
 Pseudomonas putida
5.1.2.2 salicylate reversible Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.2.2 0.63
-
 D-mandelate
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.2.2 Co2+ Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement Pseudomonas putida
5.1.2.2 Co2+ absolute requirement for a divalent metal ion Pseudomonas putida
5.1.2.2 Fe2+ absolute requirement for a divalent metal ion: Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ Pseudomonas putida
5.1.2.2 Mg2+ Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement Pseudomonas putida
5.1.2.2 Mg2+ absolute requirement for a divalent metal ion Pseudomonas putida
5.1.2.2 Mn2+ Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement Pseudomonas putida
5.1.2.2 Mn2+ absolute requirement for a divalent metal ion Pseudomonas putida
5.1.2.2 Ni2+ Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement Pseudomonas putida
5.1.2.2 Ni2+ absolute requirement for a divalent metal ion Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.2.2 69500
-
 4 * 69500, SDS-PAGE Pseudomonas putida
5.1.2.2 278000
-
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.2.2 D-mandelate Pseudomonas putida strictly inducible enzyme. Inducers: D-mandelate, L-mandelate, benzoylformate L-mandelate
-
 ?
5.1.2.2 D-mandelate Pseudomonas putida first enzyme of pathway of oxidation of D,L-mandelate that permits certain strains of Pseudomonas putida to use mandelate as a sole source of carbon and energy L-mandelate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.2.2 Pseudomonas putida
-
 biotype A, ATCC 12633, this strain is also known as A.3.12 and PRS
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.1.2.2 (S)-mandelate = (R)-mandelate pathway involving a carbanion intermediate Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.2.2 D-mandelate
-
 Pseudomonas putida L-mandelate
-
 ?
5.1.2.2 D-mandelate strictly inducible enzyme. Inducers: D-mandelate, L-mandelate, benzoylformate Pseudomonas putida L-mandelate
-
 ?
5.1.2.2 D-mandelate first enzyme of pathway of oxidation of D,L-mandelate that permits certain strains of Pseudomonas putida to use mandelate as a sole source of carbon and energy Pseudomonas putida L-mandelate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.2.2 tetramer 4 * 69500, SDS-PAGE Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.2.2 1070
-
 D-mandelate
-
 Pseudomonas putida