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Literature summary extracted from
- Glycolytic enzymes of Trypanosoma brucei. Simultaneous purification, intraglycosomal concentrations and physical properties (1986), Eur. J. Biochem., 157, 441-453.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.3.1.9 | glycosome | - |
Trypanosoma brucei | 20015 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 69000 | - |
66000-72000 Da, gel filtration | Trypanosoma brucei |
| 5.3.1.9 | 105000 | - |
gel filtration | Trypanosoma brucei |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Trypanosoma brucei | - |
dihydroxyacetone phosphate + NADH + H+ | - |
? |  |
| 5.3.1.9 | additional information | Trypanosoma brucei | glycolytic enzyme | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.8 | Trypanosoma brucei | - |
- |
- |
| 5.3.1.9 | Trypanosoma brucei | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.8 | - |
Trypanosoma brucei |
| 5.3.1.9 | - |
Trypanosoma brucei |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 170 | - |
standard deviation 45 units/mg | Trypanosoma brucei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Trypanosoma brucei | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
| 5.3.1.9 | additional information | glycolytic enzyme | Trypanosoma brucei | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.8 | dimer | 2 * 37200-37900 | Trypanosoma brucei |
| 5.3.1.9 | ? | - |
Trypanosoma brucei |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.8 | NAD+ | - |
Trypanosoma brucei | |
| 1.1.1.8 | NADH | - |
Trypanosoma brucei | |
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Release 2023.1 (January 2023)
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