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Literature summary extracted from

  • Tisljar, U.; Wolf, D.H.
    Purification and characterization of the cystinyl bond cleaving yeast aminopeptidase yscXVI (1993), FEBS Lett., 322, 191-196.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.3 amastatin
-
Saccharomyces cerevisiae
3.4.11.3 bestatin
-
Saccharomyces cerevisiae
3.4.11.22 1,10 phenanthroline
-
Saccharomyces cerevisiae
3.4.11.22 amastatin
-
Saccharomyces cerevisiae
3.4.11.22 bestatin
-
Saccharomyces cerevisiae
3.4.11.22 EDTA
-
Saccharomyces cerevisiae
3.4.11.22 additional information no inhibition by phenylmethylsulfonyl fluoride, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, N.ethylmaleimide, pepstatin, phosphoramidon, puromycin Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.3 0.0225
-
L-cysteine-nitroanilide
-
Saccharomyces cerevisiae
3.4.11.22 0.0225
-
Cystinyl 4-nitroanilide
-
Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.3 Co2+ reverses EDTA inhibition Saccharomyces cerevisiae
3.4.11.3 Mn2+ reversal of EDTA inhibition, inhibition at high concentration Saccharomyces cerevisiae
3.4.11.3 Zn2+ reverses EDTA inhibition Saccharomyces cerevisiae
3.4.11.22 Co2+ CoCl2 activates Saccharomyces cerevisiae
3.4.11.22 Zn2+ ZnCl2 has no influence on activity Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.3 52000
-
non-denaturing PAGE Saccharomyces cerevisiae
3.4.11.22 45000
-
x * 45000, SDS-PAGE Saccharomyces cerevisiae
3.4.11.22 52000
-
gel filtration, non-denaturing PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.3 Saccharomyces cerevisiae
-
-
-
3.4.11.22 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.22 no glycoprotein no N-glycosylation Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.3
-
Saccharomyces cerevisiae
3.4.11.22 aminopeptidase yscXVI Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.3 0.0129
-
-
Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
3.4.11.22 4°C, 50 mM Tris-HCl buffer, pH 7.2, 38% loss of activity after 4 days, 81% loss of activity after 11 days Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.3 Cys-4-nitroanilide + H2O
-
Saccharomyces cerevisiae Cys + 4-nitroaniline
-
?
3.4.11.3 L-cystine-di-p-nitroanilide + H2O
-
Saccharomyces cerevisiae L-cystine + p-nitroaniline
-
?
3.4.11.3 L-lysine-p-nitroanilide + H2O
-
Saccharomyces cerevisiae L-lysine + p-nitroaniline
-
?
3.4.11.22 Ala 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Ala + 4-nitroaniline
-
?
3.4.11.22 Arg 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Arg + 4-nitroaniline
-
?
3.4.11.22 Cystinyl 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Cystine + 4-nitroaniline
-
?
3.4.11.22 Glu 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Glu + 4-nitroaniline
-
?
3.4.11.22 Gly 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Gly + 4-nitroaniline
-
?
3.4.11.22 Leu 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Leu + 4-nitroaniline
-
?
3.4.11.22 Lys 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Lys + 4-nitroaniline
-
?
3.4.11.22 Met 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Met + 4-nitroaniline
-
?
3.4.11.22 additional information no hydrolyis of succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, succinyl-Ala-Ala-Pro-Leu 4-nitroanilide, succinyl-Ala-Ala-Val- Ala 4-nitroanilide, benzyloxycarbonyl-Arg-Arg 4-nitroanilide, benzyloxycarbonyl-Gly-Pro 4-nitroanilide, benzyloxycarbonyl-Pro-Phe-Arg 4-nitroanilide, acetyl-Arg-Gly-Gly-Met 4-nitroanilide, benzoyl-Ile-Glu-Gly-Arg 4-nitroanilide, benzoyl-Tyr 4-nitroanilide, benzyloxycarbonyl-Lys-Arg 4-nitroanilide Saccharomyces cerevisiae ?
-
?
3.4.11.22 Phe 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Phe + 4-nitroaniline
-
?
3.4.11.22 Pro 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Pro + 4-nitroaniline
-
?
3.4.11.22 S-Benzoyl-Cys 4-nitroanilide + H2O
-
Saccharomyces cerevisiae S-Benzoyl-Cys + 4-nitroaniline
-
?
3.4.11.22 Val 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Val + 4-nitroaniline
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.22 ? x * 45000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.22 7 8 substrate cystinyl 4-nitroanilide Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.22 4 9 no activity at pH 4 and pH 9, about 33% of maximal activity at pH 6 Saccharomyces cerevisiae