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Literature summary extracted from

  • Bernard, A.R.; Wells, T.N.C.; Cleasby, A.; Borlat, F.; Payton, M.A.; Proufoot, A.E.I.
    Selenomethionine labelling phosphomannose isomerase changes its kinetic properties (1995), Eur. J. Biochem., 230, 111-118.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.3.1.8 pharmacology absence of mannose-6-phosphate isomerase causes cell lysis and thus the enzyme is a potential target for inhibition and may be a route to antifungal drugs Candida albicans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.8 wild-type and selenomethionine-labelled enzyme expressed in Escherichia coli Candida albicans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.8
-
 Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.8 Cd2+
-
 Candida albicans
5.3.1.8 Hg2+ recombinant wild-type enzyme and selenomethionine-labelled enzyme Candida albicans
5.3.1.8 Zn2+
-
 Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.8 0.2
-
 mannose 6-phosphate Hepes buffer Candida albicans
5.3.1.8 0.8
-
 mannose 6-phosphate selenomethionine-labelled enzyme, Hepes buffer Candida albicans
5.3.1.8 1
-
 mannose 6-phosphate Tris/HCl buffer Candida albicans
5.3.1.8 4
-
 mannose 6-phosphate selenomethionine-labelled enzyme, Tris/HCl buffer Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.8 48740
-
 wild-type recombinant enzyme, electrospray mass spectroscopy Candida albicans
5.3.1.8 49060
-
 selenomethionine-labelled enzyme expressed in E. coli, electrospray mass spectroscopy Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.8 Candida albicans
-
 wild-type and selenomethionine-labelled enzyme expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.8 normal and selenomethionine-labelled enzyme expressed in Escherichia coli Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.8 additional information
-
-
 Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.8 D-Mannose 6-phosphate
-
 Candida albicans D-Fructose 6-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.8 121
-
 mannose 6-phosphate selenomethionine-labelled enzyme Candida albicans
5.3.1.8 162
-
 mannose 6-phosphate wild-type enzyme Candida albicans