BRENDA - Enzyme Database

Studies on the phosphomannose isomerase of Amorphophallus konjac C. Koch. I. Its isolation and some enzymic properties

Murata, T.; Plant Cell Physiol. 16, 953-961 (1975)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.1.8
1,10-phenanthroline
-
Amorphophallus konjac
5.3.1.8
2,2'-dipyridyl
-
Amorphophallus konjac
5.3.1.8
EDTA
-
Amorphophallus konjac
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.1.8
0.73
-
mannose 6-phosphate
-
Amorphophallus konjac
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.1.8
45000
-
gel filtration
Amorphophallus konjac
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.1.8
Amorphophallus konjac
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.1.8
-
Amorphophallus konjac
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.1.8
corm
-
Amorphophallus konjac
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.1.8
5.95
-
-
Amorphophallus konjac
Storage Stability
EC Number
Storage Stability
Organism
5.3.1.8
-20°C, unstable
Amorphophallus konjac
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.1.8
D-Mannose 6-phosphate
-
2810
Amorphophallus konjac
D-Fructose 6-phosphate
-
2810
Amorphophallus konjac
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5.3.1.8
40
-
pH 10, about 25% loss of activity; pH 5.5, 1 h, about 35% loss of activity; pH 6.0-9.5, 1 h, no loss of activity
Amorphophallus konjac
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.1.8
6.5
7
-
Amorphophallus konjac
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.3.1.8
5.5
9
about 50% of maximal activity at pH 5.5 and pH 9.0
Amorphophallus konjac
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
5.3.1.8
5.5
-
40°C, 1 h, about 35% loss of activity
Amorphophallus konjac
5.3.1.8
6
9.5
40°C, 1 h, no loss of activity
Amorphophallus konjac
5.3.1.8
10
-
40°C, 1 h, about 25% loss of activity
Amorphophallus konjac
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.1.8
1,10-phenanthroline
-
Amorphophallus konjac
5.3.1.8
2,2'-dipyridyl
-
Amorphophallus konjac
5.3.1.8
EDTA
-
Amorphophallus konjac
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.1.8
0.73
-
mannose 6-phosphate
-
Amorphophallus konjac
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.1.8
45000
-
gel filtration
Amorphophallus konjac
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.1.8
-
Amorphophallus konjac
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.1.8
corm
-
Amorphophallus konjac
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.1.8
5.95
-
-
Amorphophallus konjac
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
5.3.1.8
-20°C, unstable
Amorphophallus konjac
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.1.8
D-Mannose 6-phosphate
-
2810
Amorphophallus konjac
D-Fructose 6-phosphate
-
2810
Amorphophallus konjac
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5.3.1.8
40
-
pH 10, about 25% loss of activity; pH 5.5, 1 h, about 35% loss of activity; pH 6.0-9.5, 1 h, no loss of activity
Amorphophallus konjac
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.1.8
6.5
7
-
Amorphophallus konjac
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.3.1.8
5.5
9
about 50% of maximal activity at pH 5.5 and pH 9.0
Amorphophallus konjac
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
5.3.1.8
5.5
-
40°C, 1 h, about 35% loss of activity
Amorphophallus konjac
5.3.1.8
6
9.5
40°C, 1 h, no loss of activity
Amorphophallus konjac
5.3.1.8
10
-
40°C, 1 h, about 25% loss of activity
Amorphophallus konjac