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Literature summary extracted from

  • Carias, J.R.; Mouricout, M.; Quintard, B.; Thomes, J.C.; Julien, R.
    Leucyl-tRNA and arginyl-tRNA synthetases of wheat germ. Inactivation and ribosome effects (1978), Eur. J. Biochem., 87, 583-590.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.19 presence of ribosomes or of high concentrations of albumin oppose the conformational change of arginyl-tRNA synthetase which results in a loss of activity when the enzyme is incubated at low concentrations Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 additional information
-
 additional information
-
 Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 63000
-
 2 * 63000, gel electrophoresis, after dissociation, the enzyme exhibits an equilibrium between an active dimeric form and an inactive monomeric form Triticum aestivum
6.1.1.4 105000
-
 gel filtration Triticum aestivum
6.1.1.19 70000
-
 gel filtration Triticum aestivum
6.1.1.19 73000
-
 electrophoresis in variably cross-linked gels Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Triticum aestivum
-
-
-
6.1.1.19 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.19
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.4 germ
-
 Triticum aestivum
-
6.1.1.19 germ embryos Triticum aestivum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Triticum aestivum AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 additional information leucine-dependent ATP-diphosphate exchange, leucine + ATP + enzyme/Ile-AMP-enzyme + diphosphate Triticum aestivum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 dimer 2 * 63000, gel electrophoresis, after dissociation, the enzyme exhibits an equilibrium between an active dimeric form and an inactive monomeric form Triticum aestivum
6.1.1.19 monomer 1 * 70000, PAGE after dissociation Triticum aestivum