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Literature summary extracted from
- Purification and chemical properities of mouse liver lysosomal (L form) beta-glucuronidase (1975), J. Biol. Chem., 250, 8503-8509.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.31 | freezing/thawing, no inactivation | Mus musculus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.31 | 250000 | 310000 | mouse, gel filtration | Mus musculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.31 | Mus musculus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.31 | glycoprotein | - |
Mus musculus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.31 | - |
Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.31 | liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.31 | additional information | - |
- |
Mus musculus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.31 | -20°C or 4°C, 0.02 M sodium acetate buffer, pH 5.2, 0.15 M NaCl, 1 mg/ml protein, several months, sucrose prevents aggregation | Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.31 | 4-nitrophenyl beta-D-glucuronide + H2O | - |
Mus musculus | 4-nitrophenol + beta-D-glucuronic acid | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.31 | tetramer | mouse, SDS-PAGE | Mus musculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.31 | 71 | - |
30 min, pH 5.0, 80% activity, 30 min, pH 8.0, 20% activity | Mus musculus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.31 | 200 | - |
4-nitrophenyl beta-D-glucuronide | - |
Mus musculus | |
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