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Literature summary extracted from

  • Diez, T.; Cabezas, J.A.
    Properties of two molecular forms of beta-glucuronidase from the mollusc Littorina littorea L (1979), Eur. J. Biochem., 93, 301-311.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.31 freezing, inactivation due to dissociation Littorina littorea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.31 ascorbic acid
-
 Littorina littorea
3.2.1.31 Cu2+
-
 Littorina littorea
3.2.1.31 D-glucaro-1,4-lactone
-
 Littorina littorea
3.2.1.31 glucuronic acid
-
 Littorina littorea
3.2.1.31 heparin
-
 Littorina littorea
3.2.1.31 Hg2+
-
 Littorina littorea
3.2.1.31 Ni2+
-
 Littorina littorea
3.2.1.31 Tris
-
 Littorina littorea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.31 0.018 3.08 phenolphthalein beta-D-glucuronide dependency on form of enzyme Littorina littorea
3.2.1.31 0.041 1.3 4-methylumbelliferyl beta-D-glucuronide
-
 Littorina littorea
3.2.1.31 0.13 2.9 p-nitrophenyl-beta-D-glucuronide
-
 Littorina littorea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.31 113500
-
 sedimentation equilibrium centrifugation of form I Littorina littorea
3.2.1.31 250000 310000 Littorina littorea, gel filtration Littorina littorea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.31 additional information Littorina littorea
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Littorina littorea
-
 marine mollusc
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.31 2 forms Littorina littorea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.31 liver
-
 Littorina littorea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.31 additional information
-
-
 Littorina littorea

Storage Stability

EC Number Storage Stability Organism
3.2.1.31 4°C, at least 1 month Littorina littorea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 4-methylumbelliferyl beta-D-glucuronide + H2O
-
 Littorina littorea 4-methylumbelliferone + beta-D-glucuronic acid
-
 ?
3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O
-
 Littorina littorea 4-nitrophenol + D-glucuronic acid
-
 ?
3.2.1.31 additional information
-
 Littorina littorea ?
-
 ?
3.2.1.31 phenolphthalein beta-D-glucuronide + H2O
-
 Littorina littorea phenolphthalein + D-glucuronic acid
-
 ?
3.2.1.31 phenolphthalein-beta-D-glucuronide + H2O
-
 Littorina littorea phenolphthalein + D-glucuronic acid
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.31 60
-
 form I: 60 min, form II: 5 min Littorina littorea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 3.4
-
 form II Littorina littorea
3.2.1.31 4.1
-
 form II Littorina littorea
3.2.1.31 4.4
-
 form I Littorina littorea