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Literature summary extracted from

  • Gowers, H.M.; Breuer, H.
    Purification and properties of a beta-glucuronidase from pig kidney (1980), J. Steroid Biochem., 13, 1021-1027.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.31 bovine serum albumin activation Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.31 Hg2+ reversed by EDTA Sus scrofa
3.2.1.31 Phenolic and alcoholic glucuronides
-
 Sus scrofa
3.2.1.31 sodium desoxycholate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.31 0.02
-
 oestrone-3-glucuronide
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.31 lysosome
-
 Sus scrofa 5764
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.31 Ca2+
-
 Sus scrofa
3.2.1.31 Mg2+
-
 Sus scrofa
3.2.1.31 NaCl
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.31 250000 310000 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.31 oestrone 3-glucuronide + H2O Sus scrofa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.31 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.31 additional information
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
3.2.1.31 0°C, 0.2 M potassium phosphate buffer, pH 8.0, 3 days, unstable at -20°C or 4°C or in sodium acetate buffer pH 4.0 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 oestrone 3-glucuronide + H2O
-
 Sus scrofa ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.31 56
-
-
 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.31 37
-
 presence of oestrone-3-glucuronide, 45 min Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 4 4.4 acetate buffer Sus scrofa
3.2.1.31 5.4
-
 citrate buffer Sus scrofa